Anti-MYL12A Polyclonal Antibody for WB - P19105

Core Product Specifications and Parameters

Biological Background: MYL12A Function and Localization

• MYL12A encodes the myosin regulatory light chain 12A, a critical subunit of the myosin II motor protein complex that drives contractility in smooth muscle and nonmuscle cells.
• Also designated MLC-2B, MRLC3, and epididymis secretory protein Li 24 (HEL-S-24), reflecting its expression in diverse tissues.
• Regulates actomyosin-based contraction through reversible phosphorylation, which controls its interaction with the myosin heavy chain and actin.
• Phosphorylation of MYL12A is essential for fundamental cellular activities including cytokinesis, receptor capping, and cell migration.
• Contains calcium-binding EF-hand motifs, placing it within the family of metal-binding motor proteins.
• Predicted molecular weight of 19794 Da (approximately 20 kDa) by sequence analysis, consistent with observed electrophoretic mobility.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole-cell lysates from human cell lines such as HeLa or Jurkat as positive controls; the expected band size is ~20 kDa.
• Optimize antibody dilution starting from 1:500 to 1:2000 for WB; due to polyclonal nature, perform individual lot testing.
• For ELISA, use the recombinant immunogen (aa 92-171) as a coating antigen; assess cross-reactivity with other myosin light chain family members.
• Include phospho‑specific detection if studying signaling events, as MYL12A activity is phosphorylation‑dependent.
• Validate specificity in knockdown or knockout models if available, to confirm target recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic development by offering this anti‑MYL12A polyclonal antibody alongside a comprehensive portfolio of IVD raw materials. Whether you need validated antibody pairs, bulk ancillary reagents, or rare‑target sourcing, we provide efficient procurement solutions to accelerate your assay development in the field of cytoskeletal biology and contractile research.