Anti-MYL1 Polyclonal Antibody for WB, ELISA - P05976

Core Product Specifications and Parameters

Biological Background: MYL1 Function and Localization

• MYL1 encodes the skeletal muscle isoform of myosin light chain 1/3, also referred to as myosin light chain alkali 1/2.
• It functions as a non-regulatory myosin light chain essential for proper formation and/or maintenance of myofibers, ensuring appropriate muscle function.
• Alternative splicing of MYL1 produces multiple isoforms, contributing to the diversity of myosin light chains in skeletal muscle.
• Post-translational modifications, including acetylation, methylation, and phosphorylation, are indicated for this protein.
• The protein is a component of the myosin motor complex, participating in muscle contraction mechanisms.
• Genetic variants in MYL1 are associated with disease phenotypes, highlighting its clinical significance.
• Proteomics identification confirms its reliable detection in mass spectrometry-based protein studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–150) of human MYL1, which is conserved across known isoforms; consider this when assessing isoform reactivity.
• For Western blot, a band near 21 kDa is expected; optimize gel percentage and transfer conditions for low-molecular-weight proteins.
• ELISA protocols may require optimization of antibody concentration to achieve desired sensitivity and specificity.
• Cross-reactivity is validated for human, mouse, and rat; for other species, sequence homology should be assessed and experimental validation is recommended.
• The antibody may recognize both MLC1 and MLC3 isoforms due to the shared N-terminal sequence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for IVD raw materials, from antibodies and antigens to technical consulting, supporting the entire developmental path from concept to clinic. For skeletal muscle and myopathy-related research, we offer validated MYL1 antibodies, matched antibody pairs, and bulk ancillary reagents tailored to diagnostic assay development. Contact our team to explore customized sourcing strategies and accelerate your project timeline.