Anti-MYH9 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P35579

Core Product Specifications and Parameters

Biological Background: MYH9 Function and Localization

• Myosin-9 (MYH9) is a non-muscle myosin heavy chain, also designated NMMHC-IIA, NMHC-II-A, or cellular myosin heavy chain type A.
• It is essential for cytokinesis, cell shape maintenance, and specialized functions such as secretion and capping.
• MYH9 promotes cell motility by cooperating with S100A4. Related references: PMID:16707441
• During cell spreading, MYH9 is critical for cytoskeleton reorganization, focal contact formation at the margins, and lamellipodial retraction; its function is mechanically antagonized by MYH10. Related references: PMID:20052411
• Subcellular localization includes the cytoplasm, cell cortex, cytoplasmic vesicles, secretory vesicles, and cell membrane.
• It is expressed in the kidney glomeruli and leukocytes.
• MYH9 acts as a receptor for herpes simplex virus 1 (HSV-1) envelope glycoprotein B, facilitating viral entry.
• Post-translational modifications and functional features include acetylation, phosphorylation, ATP-binding, actin-binding, and calmodulin-binding; it is associated with diseases like Alport syndrome, deafness, and cataract.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1711-1960 near the C-terminus of human MYH9; thus, the antibody may recognize full-length MYH9 and possibly some isoforms. Validate specificity in your model system.
• For western blotting, the predicted molecular weight is ~227 kDa; consider using a low-percentage gel (e.g., 6–8% SDS-PAGE) and optimized transfer conditions for high-molecular-weight proteins.
• In immunohistochemistry (IHC-P), thorough antigen retrieval may enhance signal; test different retrieval buffers to optimize staining.
• ELISA can be employed for quantitative detection; include appropriate positive and negative controls to assess background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and researchers, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. This MYH9 antibody supports research in cell motility, cytoskeletal dynamics, and related disease areas. From concept to clinic, CamelBio bridges your supply chain with a comprehensive portfolio and technical consulting.