Anti-MYH7/β-MHC Monoclonal Antibody for WB, ELISA - P12883

Core Product Specifications and Parameters

Biological Background: MYH7/β-MHC Function and Localization

• Myosin-7 is an actin-based motor protein with ATPase activity, essential for muscle contraction.
• It forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.
• Belongs to the myosin heavy chain family, specifically the cardiac muscle beta isoform (MyHC-beta).
• Localizes to the cytoplasm, myofibrils, and sarcomeres.
• Detected in skeletal muscle at the protein level.
• Post-translational modifications include methylation and phosphorylation.
• Binds actin, ATP, and calmodulin.
• Variants are associated with cardiomyopathy and other muscle diseases.

Experimental Guidance and Technical Tips

• The immunogen covers a C-terminal region (aa 1700–1800) of human MYH7/β-MHC, which may influence epitope accessibility. For WB, consider using reduced-denatured samples, given the predicted 223 kDa molecular weight.
• Validate antibody specificity by knockdown or overexpression experiments in a relevant cell line or tissue.
• For ELISA, titrate the antibody against the immunogen peptide or recombinant MYH7 protein to determine optimal working concentrations.
• Cross-reactivity with mouse and rat MYH7 is expected based on sequence homology; verify performance in the intended species and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-MYH7/β-MHC monoclonal antibody alongside a comprehensive portfolio of validated IVD raw materials, including optimized antibody pairs, bulk ancillary reagents, and rare-target sourcing for cardiac and skeletal muscle research. Our integrated supply chain supports diagnostic manufacturers and laboratories from early-stage development through to clinical application, ensuring reliable access to critical components.