Anti-MYH7B Rabbit Monoclonal Antibody for WB, ELISA - A7E2Y1

Core Product Specifications and Parameters

Biological Background: MYH7B Function and Localization

• MYH7B encodes myosin-7B, also known as myosin heavy chain 7B, cardiac muscle beta isoform, or MYH14, a member of the myosin superfamily of motor proteins.
• It functions as an ATP-dependent motor protein involved in muscle contraction, interacting with actin filaments to generate force and movement.
• The protein contains characteristic myosin domains, including an N-terminal motor head with ATP- and actin-binding sites, a neck region, and a C-terminal coiled-coil tail that mediates dimerization and thick filament formation.
• Subcellularly, MYH7B localizes to the membrane, consistent with its role in the sarcomere structure of muscle cells.
• MYH7B exhibits tissue-specific expression, with highest levels in heart, skeletal muscle, testis, and brain; moderate expression in lung, pancreas, spleen, and liver; and lower expression in ovary and kidney.
• The gene undergoes alternative splicing, generating multiple isoforms that may have distinct functional properties or tissue distributions.
• Key post-translational modifications and binding properties include actin-binding, ATP-binding, and nucleotide-binding, essential for its mechanochemical cycle.
• As a reference proteome component and proteomics identification target, MYH7B serves as a reliable marker for cardiac and skeletal muscle research.

Experimental Guidance and Technical Tips

• For Western blot, the expected molecular weight is approximately 226 kDa; consider using low-percentage polyacrylamide gels (e.g., 6% resolving gel) and extended transfer times to ensure efficient transfer of this high-molecular-weight protein.
• The immunogen corresponds to the C-terminal region (amino acids 1750–1983); the antibody may recognize epitopes in this domain, which can influence experimental design for blocking or epitope mapping.
• Cross-reactivity with mouse and rat indicates conserved epitope sequences; it is advisable to validate detection sensitivity and specificity in the relevant sample systems when using these species.
• For ELISA, optimize coating antigen concentration and antibody dilution; this monoclonal antibody may be suitable for use as a detection antibody in sandwich ELISA when paired with a capture antibody targeting a non-overlapping epitope.
• Always include appropriate positive controls (e.g., recombinant MYH7B protein or tissue lysates from heart/skeletal muscle) and negative controls (e.g., knockout or knockdown samples) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive procurement platform for IVD raw materials, including this MYH7B rabbit monoclonal antibody, to support your research and diagnostic development in muscle biology and cardiac function. Our sourcing capabilities encompass validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized rare target raw-material supply to ensure you have reliable, scalable access to critical reagents from concept to clinic.