Anti-MYH7 Rabbit Polyclonal Antibody - P12883

Core Product Specifications and Parameters

Biological Background: Myosin-7 Function and Localization

• Myosin-7 (MYH7) is the beta isoform of myosin heavy chain, also known as myosin heavy chain slow isoform (MyHC-slow) and cardiac muscle beta isoform (MyHC-beta). It is encoded by the MYH7 gene in humans.
• As a classic myosin motor protein, MYH7 possesses actin-binding and ATPase activities, driving the sliding of actin filaments to generate contractile force in cardiac and slow skeletal muscle.
• Together with actin thin filaments, MYH7 forms regular bipolar thick filaments, constituting the fundamental contractile unit of sarcomeres in muscle cells.
• Located in the cytoplasm, specifically within myofibrils and sarcomeres, MYH7 is a key component of the sarcomere thick filament.
• Both wild-type MYH7 and its variant Gln-403 are detected at the protein level in skeletal muscle, indicating expression beyond cardiac tissue.
• Post-translational modifications include phosphorylation and methylation, and mutations in MYH7 are associated with cardiomyopathy, underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1240–1340 of human MYH7. This region is likely within the tail domain, which may help generate antibodies recognizing native and denatured MYH7.
• For Western blotting (WB), a strong band corresponding to the ~223 kDa myosin heavy chain is expected in protein extracts from heart and slow skeletal muscle tissues. Optimize protein loading and transfer conditions due to the high molecular weight.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), the antibody should label sarcomeric structures in cardiac and skeletal muscle sections. Use appropriate fixation (e.g., paraformaldehyde) and permeabilization.
• ELISA can be used for antibody titration and specificity testing against the immunogenic peptide.
• Validate cross-reactivity with mouse and rat samples using tissue extracts or sections; the high sequence conservation among mammals supports likely detection across these species.
• As with any polyclonal antibody, lot-to-lot variability should be considered, and researchers may need to optimize dilution factors for their specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, clinical laboratories, and research institutes a streamlined one-stop sourcing solution for IVD raw materials. This MYH7 rabbit polyclonal antibody supports muscle biology and cardiovascular disease research, integrating with our comprehensive portfolio of validated monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. With expertise spanning concept to clinic, CamelBio accelerates your assay development and production timelines.