Anti-MYH6/MYH7 Monoclonal Antibody for WB, IF-P, ELISA - P13533 / P12883

Core Product Specifications and Parameters

Biological Background: MYH6/MYH7 Function and Localization

• MYH6 encodes the alpha cardiac myosin heavy chain (MyHC-α), while MYH7 encodes the beta isoform (MyHC-β), both essential motor proteins in cardiac sarcomeres.
• Myosin-6 converts chemical energy from ATP hydrolysis into mechanical force, driving muscle contraction by sliding actin filaments.
• The protein features an N-terminal motor domain with ATPase activity and actin-binding site, and a C-terminal coiled‑coil tail for thick filament assembly.
• Localized to the cytoplasm, specifically within myofibrils of cardiac muscle cells, contributing to the contractile machinery.
• Post‑translational modifications, including phosphorylation and methylation, regulate motor function and filament organization.
• Genetic variants in MYH6 or MYH7 are linked to inherited conditions such as hypertrophic cardiomyopathy and atrial septal defects.

Experimental Guidance and Technical Tips

• For Western blot, expect a band at ~223 kDa; use a low‑percentage SDS‑PAGE and optimized transfer conditions for high‑molecular‑weight proteins.
• In immunofluorescence, permeabilize cells to detect the myofibrillar cytoplasmic distribution; a pattern of striated staining is expected in cardiomyocytes.
• The immunogen lies within a region common to both MYH6 and MYH7, so the antibody recognizes both isoforms; verify specificity in your model system using knockout controls or isoform‑specific detection if needed.
• For ELISA, pair with a suitable detection antibody and validate against recombinant proteins or native tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement of high‑quality IVD raw materials, including validated antibody pairs and monoclonal antibodies for cardiac biomarker development. We offer bulk reagents, ancillary buffers, and rare‑target sourcing to support your assay from concept to clinic. Contact us to explore MYH6/MYH7‑related raw materials tailored to your cardiovascular diagnostic application.