Anti-MYH6/α-MHC Rabbit Polyclonal Antibody - Q02566

Core Product Specifications and Parameters

Biological Background: MYH6/α-MHC Function and Localization

• MYH6 encodes the alpha heavy chain subunit of cardiac myosin, a major contractile protein in heart muscle.
• Myosin-6 is an actin-based motor protein with ATPase activity, essential for converting chemical energy into mechanical force during muscle contraction.
• Localized to the cytoplasm and specifically to myofibrils in cardiac muscle cells, where it forms thick filaments of the sarcomere.
• The protein contains coiled-coil domains for dimerization and an N-terminal motor domain with actin- and ATP-binding sites.
• Post-translational modifications include methylation and phosphorylation, which may regulate myosin function and filament assembly.
• This antibody recognizes the alpha isoform of cardiac myosin heavy chain, which is distinct from the beta isoform (MYH7) expressed in slow skeletal muscle and ventricles under certain conditions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to a sequence within amino acids 621-720 of mouse MYH6/α-MHC. This region may be conserved, allowing cross-reactivity with rat as indicated.
• For Western blotting (WB), a band around 224 kDa is expected. Use appropriate gel percentage and transfer conditions for high molecular weight proteins.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), fixation and permeabilization should be optimized for thick filament detection in cardiac tissue.
• For immunoprecipitation (IP), note that myosin forms large complexes; gentle lysis and inclusion of ATP may assist in preserving interactions.
• ELISA can be used for quantification or screening; consider coating with the immunogen peptide or purified protein.
• Always include appropriate controls and validate the antibody in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with one-stop access to validated IVD raw materials, including this anti-MYH6/α-MHC antibody for cardiac muscle research. Our portfolio covers optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing of rare-target materials to streamline your assay development from concept to clinic.