Anti-MYH4/MYH2B Rabbit Monoclonal Antibody for WB - Q9Y623

Core Product Specifications and Parameters

Biological Background: MYH4 Function and Localization

• Myosin-4 (MYH4), also designated MyHC-IIb or MYH2B, is a class II myosin heavy chain predominantly expressed in fast-twitch skeletal muscle fibers.
• As a molecular motor, MYH4 drives muscle contraction by ATP-dependent sliding of actin filaments, essential for force generation in striated muscles.
• MYH4 belongs to the myosin heavy chain superfamily, which includes multiple isoforms with distinct ATPase activities and contractile properties.
• The protein localizes to the cytoplasm and integrates into myofibrils, the basic contractile units of muscle cells.
• Post-translational modifications such as phosphorylation and methylation regulate MYH4 function and filament assembly, consistent with keywords like Phosphoprotein and Methylation.
• MYH4 contains key domains for actin binding, ATP binding, and calmodulin binding, forming a coiled-coil tail that mediates dimerization into thick filaments.
• Alternative names include Myosin heavy chain 2b, Myosin heavy chain 4, Myosin heavy chain IIb, and Myosin heavy chain, skeletal muscle, fetal, reflecting historical and tissue-specific nomenclature.
• The molecular weight of MYH4 is approximately 223 kDa, consistent with other large myosin heavy chains.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 1500-1600); consider epitope accessibility when designing blocking or detection strategies.
• For Western blotting, the large 223 kDa target requires careful gel handling and optimized transfer conditions to ensure efficient detection.
• When using this antibody in immunoprecipitation, note the high molecular weight of the motor protein; validate lysis and capture protocols accordingly.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), confirm specificity in relevant skeletal muscle tissue sections.
• Cross-reactivity with mouse and rat supports translational studies, but validate in the appropriate sample system due to potential isoform differences.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-MYH4 antibody supports muscle biology and myopathy research, reflecting our commitment to rare target sourcing and tailored raw-material solutions. Whether you need ready-to-use reagents or custom development, CamelBio bridges the gap from concept to clinic.