Anti-MYH4 Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9Y623

Core Product Specifications and Parameters

Biological Background: MYH4 Function and Localization

• Myosin-4 is a myosin heavy chain isoform also known as MyHC‑2b, MyHC‑IIb, or fetal myosin heavy chain, encoded by the MYH4 gene.
• It functions as a molecular motor in muscle contraction, converting ATP hydrolysis energy into mechanical force through cyclic interactions with actin filaments.
• The protein localizes to the cytoplasm and specifically incorporates into myofibrils as a major component of the thick filament.
• It contains characteristic motor protein domains: an N‑terminal motor head with ATP‑ and actin‑binding sites, a neck region with calmodulin‑binding IQ motifs, and a long coiled‑coil tail for dimerization and filament assembly.
• Post‑translational modifications include methylation and phosphorylation, which may modulate enzymatic activity, filament stability, or protein‑protein interactions.
• Expression is predominantly in skeletal muscle, where it defines a fast‑twitch fiber type (type IIb), and a fetal form has been described.
• According to UniProt keywords, the protein is also classified as an actin‑binding, nucleotide‑binding, phosphoprotein, and proteomics identification target.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C‑terminal region (aa 1500–1600); this sequence is expected to be unique to MYH4 among human myosin isoforms, but sequence alignment with mouse/rat orthologs may inform cross‑reactivity.
• For Western blotting, expect a predominant band around 223 kDa in skeletal muscle lysates; consider using gradient gels and prolonged transfer times for high‑molecular‑weight proteins.
• In immunoprecipitation‑fluorescence (IF‑P) and immunohistochemistry (IHC‑P), the antibody should label myofibrils in skeletal muscle sections. Validate in tissue samples with known MYH4 expression (e.g., fast‑twitch muscle) and include negative controls (e.g., cardiac or smooth muscle).
• Cross‑reactivity with mouse and rat is reported; however, always confirm species‑specific detection in the experimental system of interest.
• This polyclonal antibody may recognize multiple epitopes, potentially increasing sensitivity but also the risk of non‑specific bands; optimization of blocking and washing conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated sourcing partner for IVD manufacturers and research laboratories, providing streamlined access to a wide range of raw materials—including validated polyclonal antibodies like this anti‑MYH4 antibody. Our comprehensive supply capabilities cover antibody pairs, optimized monoclonals, bulk ancillary reagents, and rare‑target procurement, all tailored to support your myosin research, skeletal muscle studies, and diagnostic assay development from concept to clinic.