Anti-MYH14 Polyclonal Antibody for WB, ELISA - Q7Z406

Core Product Specifications and Parameters

Biological Background: Myosin-14 Function and Localization

• Myosin-14 (MYH14) is a non-muscle myosin heavy chain (NMHC II-C) encoded by the MYH14 gene, with alternative splicing and multiple isoforms.
• It is a cellular myosin involved in cytokinesis, cell shape maintenance, and specialized processes such as secretion and capping.
• MYH14 belongs to the myosin heavy chain family and contains conserved domains for actin-binding, ATP-binding, and calmodulin-binding, characteristic of myosin motor proteins, and forms a coiled-coil structure for dimerization.
• Tissue specificity: High expression in brain (especially corpus callosum), heart, kidney, liver, lung, small intestine, colon, and skeletal muscle; low in smooth muscle-containing organs; undetectable in thymus, spleen, placenta, and lymphocytes.
• The protein undergoes post-translational modifications including acetylation and phosphorylation.
• MYH14 is associated with diseases such as deafness (DFNA4), neuropathy, and non-syndromic deafness.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning residues 600-700 of human MYH14 (NP_079005.3); consider this region when designing validation experiments, particularly for isoform specificity.
• For Western blot (WB), a band at approximately 228 kDa is expected; optimization of electrophoresis and transfer conditions for high-molecular-weight proteins may be necessary.
• ELISA may be used for detecting MYH14 in cell or tissue lysates; appropriate controls and standard curves should be included for quantitative analysis.
• As cross-reactivity is reported with human and rat, validate performance in the relevant species and sample types before large-scale use.
• Given its role in cytokinesis and cell shape, the antibody may be useful for studying myosin-14 in cellular processes; however, functional validation in biological contexts is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop sourcing for IVD raw materials, from concept to clinic. For your myosin-14 research and diagnostic development needs, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies like this MYH14 Rabbit pAb, bulk ancillary reagents, and rare target raw-material sourcing. Contact us to streamline your procurement process.