Anti-MYH1 Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P12882

Core Product Specifications and Parameters

Biological Background: Myosin-1 Function and Localization

• Myosin-1, also referred to as myosin heavy chain 2x (MyHC-2x), is a class II myosin heavy chain encoded by the MYH1 gene.
• It constitutes a core component of the thick filaments in adult skeletal muscle sarcomeres, functioning as an actin-based motor protein that couples ATP hydrolysis to mechanical force generation.
• Uniquely, myosin-1 is essential for normal auditory function; it contributes to cochlear amplification of sound stimuli by positively regulating prestin (SLC26A5) activity and outer hair cell electromotility.
• The protein features conserved domains for actin binding, ATP binding, and calmodulin binding, and its coiled-coil domains drive assembly into bipolar thick filaments.
• Subcellular localization is predominantly cytoplasmic and myofibrillar.
• Post-translational modifications, including methylation and phosphorylation, are indicated by proteomics data and may modulate myosin-1 activity.
• Keywords associated with P12882 include Actin-binding, ATP-binding, Calmodulin-binding, Coiled coil, Cytoplasm, Hearing, Methylation, Motor protein, Muscle protein, Myosin, Nucleotide-binding, Phosphoprotein, Thick filament, underscoring its dual roles in muscle contraction and hearing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 100 amino acids of human MYH1; the resulting polyclonal antibody may recognize linear epitopes within this region.
• For Western blotting, a low-percentage or gradient polyacrylamide gel is recommended to resolve the high molecular weight target (~223 kDa), and transfer conditions should be optimized for large proteins.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), consider validating the antibody in skeletal muscle tissue sections or cochlear whole-mount preparations, as these are physiologically relevant sites of MYH1 expression.
• For ELISA applications, the polyclonal nature may provide enhanced sensitivity as a capture or detection reagent, but users should determine optimal working concentrations empirically.
• Cross-reactivity with mouse and rat MYH1 is indicated; sequence homology in the immunogen region should be verified when designing species-mismatched experiments.

CamelBio: Your One-Stop Sourcing Bridge

This anti-MYH1 antibody supports research into muscle physiology and the molecular mechanisms of hearing, areas with growing diagnostic potential. CamelBio streamlines procurement for IVD developers: we supply validated antibody pairs, bulk polyclonal and monoclonal antibodies, ancillary reagents, and can source hard-to-find raw materials for rare targets—all backed by technical consultancy to accelerate your project from concept to clinic.