Anti-Myeloperoxidase (Mpo) Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P11247

Core Product Specifications and Parameters

Biological Background: Myeloperoxidase (Mpo) Function and Localization

• Myeloperoxidase (Mpo) is a heme- and iron-containing peroxidase highly expressed in neutrophil azurophilic granules, functioning as a homodimer of glycosylated heavy and light chains linked by disulfide bonds.
• As a key effector of innate immunity, Mpo catalyzes the conversion of hydrogen peroxide and chloride ions into hypochlorous acid (HOCl), a potent microbicidal oxidant.
  Related references: PMID:11593004
• Mpo is central to the formation of neutrophil extracellular traps (NETs), a process where it translocates to the nucleus, disrupts nucleosomes, and promotes chromatin decondensation to form extracellular DNA webs that trap pathogens.
  Related references: PMID:10085024, PMID:23306200
• Direct microbicidal activity of Mpo is amplified by its ability to oxidize various substrates, generating additional reactive species such as hypobromous acid, nitrogen dioxide radical, and protein tyrosine radicals.
• Mpo is a multi-compartment protein: stored in cytoplasmic azurophilic granules, translocated to phagosomes upon infection, and actively secreted; during NETosis, it localizes to the nucleus and chromosomes.
• The enzyme's activity is calcium-dependent, and its mature form is stabilized by intrachain disulfide bonds, with post-translational modifications including N-glycosylation critical for proper folding and activity.
• Additionally, Mpo contributes to inflammatory tissue damage by chlorinating and nitrating host proteins, such as apolipoprotein A-I, thereby impairing cholesterol efflux and promoting atherosclerosis.

Experimental Guidance and Technical Tips

• The immunogen, a recombinant protein spanning amino acids 16–718 of mouse Mpo, covers almost the entire precursor sequence (excluding the signal peptide), suggesting the antibody may recognize both the pro-enzyme and processed heavy/light chains.
• For Western blotting, the predicted molecular weight is ~81 kDa for the precursor; under reducing conditions, the heavy (~60 kDa) and light (~15 kDa) chains may also be detected depending on sample processing and cell type.
• For immunofluorescence and immunohistochemistry, consider optimizing permeabilization and antigen retrieval protocols to detect Mpo in azurophilic granules (cytoplasmic), phagosomes, or during NETosis when nuclear localization occurs.
• The antibody is validated for ELISA; ensure proper coating conditions and include appropriate blocking and washing steps to minimize background.
• Cross-reactivity with human and rat Mpo has been demonstrated, but signal intensity may vary; validate dilution series in the relevant species and tissue context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated supply partner for IVD raw materials, offering seamless access to high-quality antibodies, antigens, and ancillary reagents from discovery to clinical application. Beyond this myeloperoxidase polyclonal antibody, we provide validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, supporting your research in innate immunity, inflammation, and neutrophil biology. Let us help bridge your diagnostic development from concept to clinic with reliable, one-stop procurement solutions.