Anti-Myeloperoxidase (MPO) Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P05164

Core Product Specifications and Parameters

Biological Background: Myeloperoxidase (MPO) Function and Localization

• MPO is a heme-containing peroxidase that plays a critical role in the innate immune system, primarily expressed in neutrophils and monocytes.
• It mediates the formation of neutrophil extracellular traps (NETs) by translocating to the nucleus, binding to nucleosomes, and inducing chromatin decondensation and DNA unwrapping in an ATP-independent manner. Related references: PMID:40963017
• As a peroxidase, MPO catalyzes the production of hypochlorous acid (HOCl), hypobromous acid, and other reactive oxidants from hydrogen peroxide, which are essential for microbicidal activity against a broad range of pathogens. Related references: PMID:16125131, PMID:17074761, PMID:17438335
• MPO also oxidizes nitrite to nitrogen dioxide radical, contributing to protein nitration and modulation of inflammatory signaling. Related references: PMID:10777476, PMID:8385644, PMID:9450756
• The enzyme localizes to azurophilic granules (lysosomes) of neutrophils and is released into the phagosome or extracellularly upon degranulation; it has also been detected in the nucleus and on chromosomes.
• Post-translational modifications include heme binding, calcium coordination, and N-linked glycosylation; the mature protein is a tetramer of two light chains and two heavy chains derived from a single precursor.
• MPO can chlorinate and nitrate apolipoprotein A-I (APOA1), impairing cholesterol efflux and potentially contributing to atherosclerosis. Related references: PMID:15326314, PMID:15498770, PMID:15574409
• The peroxidase activity can be inhibited by the S.aureus protein SPIN, representing an immune evasion strategy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal portion of the MPO heavy chain (amino acids 606–745), which may target a conserved region across species, consistent with reported cross-reactivity with mouse and rat.
• For Western blotting, the predicted molecular weight of MPO is approximately 84 kDa (precursor) or 59 kDa (heavy chain) and 13.5 kDa (light chain) after processing; consider optimizing sample preparation to detect both precursor and mature forms.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), validate the antibody in relevant tissue or cell systems, such as neutrophil-rich inflamed tissue or isolated blood cells, to confirm specific staining.
• Perform appropriate controls (e.g., isotype control, peptide blocking) and titrate antibody concentrations for each application to achieve an optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, reference laboratories, and research institutes with high-quality IVD raw materials for immunology and infectious disease applications. Our portfolio includes validated monoclonal and polyclonal antibodies, recombinant antigens, and ancillary reagents for developing robust MPO-based diagnostic assays. Whether you need pre-optimized antibody pairs, bulk quantities, or sourcing for niche targets, CamelBio streamlines your supply chain from concept to clinic.