Anti-Myelin Protein Zero (MPZ) Polyclonal Antibody for WB, ELISA - P25189

Core Product Specifications and Parameters

Biological Background: Myelin Protein Zero Function and Localization

• Myelin protein zero (P0) is the major structural protein of peripheral nervous system myelin, encoded by the MPZ gene. It is a single-pass type I transmembrane glycoprotein belonging to the immunoglobulin superfamily.
• P0 functions as a homophilic adhesion molecule essential for normal myelination in the peripheral nervous system. It mediates adhesion between adjacent myelin wraps and ultimately drives myelin compaction.
• Alternative splicing of the MPZ gene generates multiple isoforms, which may contribute to the fine-tuning of myelin structure and function.
• The protein is exclusively localized to the myelin membrane of Schwann cells, where it forms the major dense line through interactions of its extracellular immunoglobulin-like domain and cytoplasmic tail.
• P0 undergoes key post-translational modifications, including N-linked glycosylation, disulfide bond formation, and phosphorylation, which are critical for its adhesion properties and myelin integrity.
• Mutations in MPZ are associated with a spectrum of inherited peripheral neuropathies, including Charcot-Marie-Tooth disease type 1B, Dejerine-Sottas syndrome, and congenital hypomyelinating neuropathy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 30–153) of human MPZ, which is highly conserved across mouse and rat, supporting the observed cross-reactivity.
• For Western blot, the antibody is expected to detect a band at approximately 28 kDa (glycosylated form) in lysates from peripheral nerve tissues or Schwann cells. Consider including a deglycosylation control to confirm specificity.
• In ELISA applications, the antibody can be used to capture or detect recombinant MPZ protein or peptide fragments. Validate the assay with appropriate positive and negative controls in your sample matrix.
• When using rodent models, verify the antibody’s performance in mouse or rat sciatic nerve lysates, as cross-reactivity has been reported for these species.
• For immunohistochemistry or immunofluorescence, note that the product has not been formally tested in these applications; pilot experiments with proper fixation and permeabilization are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials for myelin biology and neurodegenerative disease studies. From validated antibody pairs and polyclonal antibodies to bulk ancillary reagents, we streamline procurement for peripheral nerve research targets like MPZ. Whether you need this anti-MPZ antibody for assay development or seek custom sourcing for rare targets, our team provides tailored support to move your project from concept to clinic.