Anti-Myelin Protein Zero (MPZ) Monoclonal Antibody for WB - P25189

Core Product Specifications and Parameters

Biological Background: Myelin Protein Zero (MPZ) Function and Localization

• Myelin protein P0 (also known as myelin peripheral protein or MPZ) is a single-pass type I membrane glycoprotein that is the major structural component of peripheral nervous system myelin.
• It functions as an adhesion molecule essential for normal myelination, mediating adhesion between adjacent myelin wraps and driving myelin compaction.
• MPZ is a member of the immunoglobulin superfamily and contains both immunoglobulin-like and extracellular domains that facilitate homophilic interactions.
• Expression of MPZ is highly tissue-specific, found exclusively in Schwann cells of the peripheral nervous system.
• The protein localizes to the cell membrane and specifically to the myelin membrane, where it forms the major dense line of compact myelin.
• Post-translational modifications include glycosylation, phosphorylation, and a disulfide bond, which are critical for its structural and adhesive functions.
• Mutations in the MPZ gene are associated with various peripheral neuropathies, including Charcot-Marie-Tooth disease type 1B (CMT1B), Dejerine-Sottas syndrome, and congenital hypomyelinating neuropathy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an intracellular region (amino acids 149–248), which may allow detection of the full-length protein in denaturing and reducing SDS-PAGE/WB conditions.
• For WB, consider using lysates from peripheral nerve tissues or Schwann cell lines (human, mouse, rat) to confirm specificity and the observed 28 kDa band.
• Given cross-reactivity with mouse and rat, this antibody is suitable for translational studies in rodent models of peripheral neuropathy and myelination.
• For ELISA, validate performance using recombinant MPZ fragments or purified protein, and optimize coating and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, streamlining the path from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, with a focus on myelination and peripheral neuropathy targets like Myelin Protein Zero (MPZ). Whether you need rare-target raw materials or reliable supply for high-throughput applications, CamelBio is your trusted sourcing partner.