Anti-Myelin Oligodendrocyte Glycoprotein Rabbit Polyclonal Antibody for WB and ELISA - Q16653

Core Product Specifications and Parameters

Biological Background: Myelin Oligodendrocyte Glycoprotein Function and Localization

• Myelin-oligodendrocyte glycoprotein (MOG) is a minor component of the myelin sheath, expressed exclusively in the central nervous system (CNS) on the surface of myelin and oligodendrocyte cytoplasmic membranes.
• MOG mediates homophilic cell-cell adhesion, contributing to the completion, compaction, and maintenance of the myelin sheath and facilitating cell-cell communication.
• The protein contains an immunoglobulin (Ig)-like domain and belongs to the immunoglobulin superfamily, capable of forming disulfide bonds that stabilize its structure.
• MOG acts as a receptor for rubella virus during infection of CNS cells.
• Alternative splicing generates multiple isoforms, including a secreted form that may have distinct functions.
• Post-translational modifications include glycosylation and signal peptide processing, with a mature molecular weight of approximately 28 kDa.
• Mutations in MOG are associated with inflammatory demyelinating diseases, and it is a key autoantigen in multiple sclerosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 30–154, which lies within the extracellular Ig-like domain of MOG, making this antibody suitable for detecting the mature, glycosylated form of the protein.
• Western blotting (WB) under reducing conditions typically reveals a band at approximately 28 kDa; use lysates from brain tissue (human, mouse, or rat) as positive controls.
• For ELISA, this polyclonal antibody can be used for direct detection of MOG or in sandwich assays; cross-adsorption may be considered to minimize background in multi-species applications.
• Ensure proper validation in the relevant experimental model, as MOG expression is restricted to the CNS and may require enrichment steps in non-neural samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with consolidated access to IVD raw materials, from validated antibody pairs and polyclonal antibodies to bulk ancillary reagents. For MOG-focused research and immunoassay development, we support sourcing of rare target raw materials, including optimized recombinant proteins and matched detection systems. Our B2B platform streamlines procurement, helping you move from concept to clinic efficiently.