Anti-Myelin Oligodendrocyte Glycoprotein Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q16653

Core Product Specifications and Parameters

Biological Background: Myelin Oligodendrocyte Glycoprotein Function and Localization

• Myelin-oligodendrocyte glycoprotein (MOG) is a minor component of the myelin sheath in the central nervous system (CNS), classified under the immunoglobulin superfamily.
• It mediates homophilic cell-cell adhesion, suggesting roles in myelin sheath compaction and maintenance (By similarity).
• MOG is expressed exclusively in the CNS, localized on the surface of myelin and the cytoplasmic membranes of oligodendrocytes.
• The protein serves as a receptor for rubella virus, facilitating viral entry during infection.
• MOG undergoes alternative splicing, generating multiple isoforms that may modulate its adhesive or receptor functions.
• Key structural features include a signal peptide, an extracellular immunoglobulin domain, and a transmembrane helix, anchoring it to the cell membrane.
• Post-translational modifications include N-linked glycosylation and a conserved disulfide bond, which is typical of immunoglobulin-like domains.
• Mutations or polymorphisms in MOG are associated with demyelinating diseases, highlighting its immunopathogenic role in conditions like multiple sclerosis.

Experimental Guidance and Technical Tips

• The synthetic peptide immunogen likely targets a linear epitope; thus, the antibody may recognize denatured protein in WB applications, but native protein recognition in IF/ICC and IHC-P should also be assessed.
• For WB, consider using whole brain or spinal cord lysates from human, mouse, or rat as positive controls, given the CNS-specific expression. A band around 28 kDa is expected.
• In IF/ICC and IHC-P, signal is anticipated on the plasma membrane of oligodendrocytes and myelin sheaths. Co-staining with oligodendrocyte markers (e.g., Olig2, CNPase) is recommended for validation.
• For ELISA, pairing this capture antibody with a different detection antibody targeting a distinct epitope is advisable for optimal sandwich immunoassay development.
• Always validate antibody reactivity in the relevant species and tissue type before scaling up for diagnostic or research use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including monoclonal and polyclonal antibodies, validated antibody pairs, and bulk ancillary reagents. For targets like Myelin Oligodendrocyte Glycoprotein, which demand high specificity and cross-species reactivity, CamelBio offers optimized sourcing solutions to streamline assay development and production. Whether you require rare target reagents or large-scale supply for clinical diagnostics, CamelBio bridges the gap from concept to commercialization with reliable, one-stop support.