Anti-Myelin Basic Protein Mouse Monoclonal Antibody for WB - P02686

Core Product Specifications and Parameters

Biological Background: Myelin Basic Protein Function and Localization

• Myelin Basic Protein (MBP) is one of the most abundant proteins in the myelin membrane of the central nervous system (CNS), playing a critical role in myelin sheath formation and stabilization.
• The classic group of MBP isoforms (isoforms 4–14) are essential for myelin structure, while smaller isoforms may contribute to remyelination in conditions like multiple sclerosis.
• Alternative splicing of the MBP gene produces a wide spectrum of isoforms, including the non-classic Golli-MBPs (isoforms 1–3), which function in early brain development and T-cell signaling.
• Post-translational modifications such as acetylation, citrullination, methylation, and phosphorylation generate diverse MBP variants, each potentially with specialized functions.
• MBP is localized to the myelin membrane and the nucleus, with different isoforms exhibiting distinct subcellular distributions.
• Tissue expression varies: classic MBP isoforms are found in the CNS and peripheral nervous system, while Golli-MBP isoforms are expressed in fetal thymus, spleen, and immune cells.
• MBP has been implicated in autoimmune encephalomyelitis, as it can induce T-cell proliferation and is a key autoantigen in multiple sclerosis.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 1–304 of human MBP (full-length classic isoform), suggesting the antibody likely recognizes a conserved linear epitope present in most MBP isoforms.
• For Western blot (WB), expect a band near 33 kDa; however, due to extensive alternative splicing and post-translational modifications, multiple bands or a smear may appear, particularly in brain lysates.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), staining should be prominent in myelinated regions of brain and spinal cord; we recommend using paraffin-embedded or frozen sections with an appropriate antigen retrieval method.
• For ELISA, the antibody can be tested against recombinant MBP or native MBP purified from brain tissue; consider pairing with a detection antibody to develop a sandwich ELISA for MBP quantification.
• Since MBP is highly conserved among mammals, cross-reactivity with mouse and rat is expected, making it suitable for rodent models of neuroinflammation and demyelination.
• Always include positive controls (e.g., human brain lysate) and negative controls (e.g., non-neuronal tissue) to confirm specificity, and optimize antibody dilution for each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, technical services, and consulting to support diagnostic manufacturers and research institutes from concept to clinic. Our portfolio includes validated antibody pairs, high-quality monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers focused on neurobiology and demyelinating diseases like multiple sclerosis, we can source rare-target antibodies and assist in custom development to meet your specific needs.