Anti-Myelin Basic Protein (MBP) Rabbit Polyclonal Antibody for WB, IHC-P, IF-P, ELISA - P02686

Core Product Specifications and Parameters

Biological Background: Myelin Basic Protein Function and Localization

• MBP is one of the most abundant protein components of central nervous system (CNS) myelin, together with proteolipid protein (PLP), and plays essential roles in myelin membrane formation and stabilization.
• Alternative splicing produces classic MBP isoforms (isoform 4–14) and non-classic Golli-MBP isoforms (isoform 1–3), each with distinct expression patterns and functions.
• The smaller classic MBP isoforms are implicated in the remyelination of denuded axons in multiple sclerosis.
• Golli-MBP isoforms are expressed in the developing brain and immune system, potentially acting as components of transcriptional complexes and in T-cell signaling pathways.
• MBP induces T-cell proliferation, linking it to autoimmune responses in diseases such as multiple sclerosis.
• Subcellularly, MBP localizes to the myelin membrane and, for some isoforms, the nucleus.
• Post-translational modifications, including acetylation, citrullination, methylation, and phosphorylation, generate a wide spectrum of isomers with specialized functions.
• Classic MBP isoforms are found in both the CNS and peripheral nervous system (PNS), whereas Golli-MBP isoforms are expressed in fetal thymus, spleen, spinal cord, and immune cell lines.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region (amino acids 80–165) of human MBP, supporting the observed cross-reactivity with mouse and rat.
• For Western blot, a band around 33 kDa may be observed; note that alternative splicing and post-translational modifications can generate multiple isoforms with varying molecular weights.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), validate the antibody in appropriate CNS or PNS tissue sections, considering the expected myelin localization.
• When performing ELISA, consider using known concentrations of recombinant MBP as standards and validate the assay in relevant biological fluids.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with a one-stop sourcing platform for IVD raw materials. Our portfolio includes validated antibody pairs, bulk ancillary reagents, and monoclonal or polyclonal antibodies for challenging targets such as MBP. With a global supply chain and dedicated technical support, we help accelerate your neuroscience and autoimmune disease projects from concept to clinic.