Anti-Myelin Basic Protein (MBP) Monoclonal Antibody for WB - P02686

Core Product Specifications and Parameters

Biological Background: Myelin Basic Protein Function and Localization

• Myelin basic protein (MBP) is a major structural component of the myelin sheath in the central nervous system. Together with proteolipid protein (PLP), classic MBP isoforms (isoforms 4–14) are the most abundant proteins in CNS myelin, contributing to membrane formation and stabilization.
• The smaller classic MBP isoforms are implicated in remyelination of demyelinated axons, a process relevant to multiple sclerosis. Non‑classic Golli‑MBP isoforms (isoforms 1–3) are expressed in the developing brain and immune system, potentially acting as components of transcriptional complexes and in T‑cell signaling.
• MBP is encoded by the MBP gene, which undergoes extensive alternative splicing to generate a wide spectrum of isoforms with distinct expression patterns and functions.
• Subcellular localization varies by isoform: classic MBPs associate with the myelin membrane, while Golli‑MBPs are also found in the nucleus.
• Post‑translational modifications include acetylation, citrullination, methylation, and extensive phosphorylation, which may regulate protein interactions and immune recognition. MBP is an autoantigen in experimental autoimmune encephalomyelitis.
• Tissue specificity: classic MBP isoforms are present in both central and peripheral nervous system myelin; Golli‑MBP isoforms are additionally expressed in fetal thymus, spleen, spinal cord, and immune cell lines.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–304 of human MBP (NP_001020272.1), covering the full‑length canonical sequence; the antibody may recognize multiple MBP isoforms. In Western blot, bands may be observed around 33 kDa as well as lower molecular weight variants due to alternative splicing.
• For immunohistochemistry (IHC‑P) and immunofluorescence (IF‑P), consider optimal fixation and antigen retrieval conditions for brain or spinal cord tissue. Validate staining specificity using knockout controls or known expression patterns.
• In ELISA applications, the antibody can be tested as a capture or detection reagent; pairing with another anti‑MBP clone may improve sensitivity. Always titrate and validate in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. This anti‑MBP monoclonal antibody is part of our neuroscience‑focused catalog, suitable for myelination studies and demyelinating disease assay development. Beyond individual products, we offer customized bulk supply, rare target sourcing, and technical consultation to accelerate your project from concept to clinic.