Anti-MYEF2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9P2K5

Core Product Specifications and Parameters

Biological Background: MYEF2 Function and Localization

• MYEF2 (Myelin Expression Factor 2) is a transcriptional repressor that negatively regulates myelin basic protein (MBP) gene expression, playing a key role in myelin formation and maintenance.
• The protein binds specifically to the proximal MB1 element (5'-TTGTCC-3') within the MBP promoter, directly inhibiting transcription.
• MYEF2 function is itself modulated by PURA (purine-rich element binding protein A), which inhibits its DNA-binding activity and repressive function (By similarity).
• Localized predominantly to the nucleus, MYEF2 exerts its gene regulatory role by interacting with chromatin.
• The protein is subject to post-translational modifications including phosphorylation, methylation, and ubiquitination, which may influence its stability and activity.
• Alternative splicing generates multiple isoforms, potentially contributing to tissue-specific regulatory functions.
• MYEF2 contains RNA-binding motifs in addition to its DNA-binding domains, suggesting possible roles in RNA processing or stability.

Experimental Guidance and Technical Tips

• This polyclonal antibody is generated from a recombinant immunogen corresponding to the N-terminal region (amino acids 1-120) of human MYEF2. It may detect multiple isoforms due to alternative splicing.
• For Western blot, a band around 64 kDa is expected in human, mouse, and rat samples. Consider using nuclear extracts to enrich the target protein.
• In immunohistochemistry (paraffin) and immunocytochemistry, nuclear staining should be observed. Validate antibody specificity in tissues or cell lines known to express MYEF2.
• The antibody can be used in ELISA for quantitative detection of MYEF2; ensure proper controls are included to assess background.
• Since MYEF2 is a transcriptional repressor, its expression may vary under different developmental or pathological conditions; optimize experimental conditions accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as an integrated sourcing partner for IVD manufacturers and research laboratories, offering a comprehensive portfolio of validated raw materials and technical support. This anti-MYEF2 polyclonal antibody supports research into myelin gene regulation and neurological disorders, and is part of our broader collection of transcription factor antibodies. In addition to this product, we can supply optimized antibody pairs, bulk ancillary reagents, and assist in sourcing rare-target raw materials to streamline your assay development and production needs.