Anti-MyD88 Rabbit PolymAb® Monoclonal Antibody for WB, IP, ELISA - Q99836

Core Product Specifications and Parameters

Biological Background: MyD88 Function and Localization

• Serves as a central adapter in Toll-like receptor and interleukin-1 receptor signaling, critical for the innate immune response. Related references: PMID:15361868 PMID:18292575 PMID:33718825
• Propagates signals through IRAK1, IRAK2, TRAF6, and IRF7, leading to NF-κB activation and inflammatory cytokine production. Related references: PMID:15361868 PMID:19506249 PMID:24316379
• Enhances IL-8 transcription and participates in IL-18-mediated pathways. Related references: PMID:9013863
• Upon recognition of viral GU-rich single-stranded RNA (e.g., SARS-CoV-2) by TLR8, MyD88 induces NLRP3 inflammasome activation and IL-1β release. Related references: PMID:33718825
• Activates IRF1, promoting its nuclear entry to drive IFN-β, NOS2, and IL12A gene expression.
• Plays a key role in intestinal epithelial homeostasis by regulating the antimicrobial lectin REG3G (By similarity).
• Localizes to both cytoplasm and nucleus; undergoes phosphorylation and ubiquitination that modulate signaling.
• Ubiquitously expressed, and linked to antiviral defense and disease variants.

Experimental Guidance and Technical Tips

• The immunogen maps to the N-terminal region (aa 50-150), which includes the Death Domain (DD) important for homotypic interactions. This antibody may recognize the DD and could be useful for studying protein-protein interactions.
• For WB, expect a band at ~33 kDa. Validate in human and mouse lysates and consider appropriate loading controls.
• For IP, confirm specificity with isotype controls and optimize lysis conditions to preserve antigen binding.
• For ELISA, empirically determine optimal working concentrations for capture or detection formats.
• MyD88 is subject to phosphorylation and ubiquitination, which may influence antibody binding; consider using phosphatase inhibitors if needed.
• Cross-reactivity with mouse MyD88 enables broad utility, but further species validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline diagnostic development. This anti-MyD88 antibody supports research into TLR/IL-1R innate immune signaling, and our team can source complementary raw materials for your pipeline. Let us simplify your procurement from concept to clinic.