Anti-MyD88 Rabbit Monoclonal Antibody [KO Validated] for WB, IHC-P, ELISA - Q99836

Core Product Specifications and Parameters

Biological Background: MyD88 Function and Localization

• Alternative splicing of the MYD88 gene produces multiple isoforms, yielding protein species ranging from 15 to 34 kDa.
• MyD88 serves as a central adaptor protein in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling, essential for innate immunity. Related references: PMID:15361868 PMID:18292575 PMID:33718825
• Through recruitment of IRAK1, IRAK2, IRF7, and TRAF6, MyD88 triggers NF-κB activation and drives pro-inflammatory cytokine secretion. Related references: PMID:15361868 PMID:19506249 PMID:24316379
• MyD88 enhances IL-8 transcription, promoting neutrophil chemotaxis and inflammatory responses. Related references: PMID:9013863
• Upon sensing viral GU-rich single-stranded RNA via TLR8, MyD88 activates the NLRP3 inflammasome, leading to IL-1β release and antiviral defense. Related references: PMID:33718825
• MyD88 is ubiquitously expressed and plays a critical role in intestinal epithelial cells, maintaining gut homeostasis by controlling expression of the antimicrobial lectin REG3G (By similarity).
• MyD88 localizes to both the cytoplasm and nucleus; its shuttling and activity are modulated by post-translational modifications such as phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50–150 of human MyD88 (NP_002459.3), suggesting the antibody likely recognizes an N-terminal region conserved across human, mouse, and rat.
• In Western blot, multiple bands between 15–34 kDa may be observed due to alternative splicing isoforms; use knockdown or knockout controls for validation.
• For IHC-P, optimize antigen retrieval conditions and include appropriate tissue controls; MyD88 is ubiquitously expressed, so positive staining is expected in most samples.
• This antibody is validated for ELISA; consider pairing with a suitable detection antibody for sandwich ELISA applications after confirming specificity in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw material sourcing, offering validated antibody pairs, mono‑ and polyclonal antibodies, bulk ancillary reagents, and custom rare‑target procurement. Our MyD88 rabbit monoclonal antibody supports innate immunity research, from TLR signaling studies to inflammatory disease diagnostics. Let CamelBio streamline your supply chain from concept to clinic with reliable, high-quality reagents and expert technical support.