Anti-MyD88 Polyclonal Antibody for WB, IF/ICC, ELISA - Q99836

Core Product Specifications and Parameters

Biological Background: MyD88 Function and Localization

• MyD88 acts as a central adaptor in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling, initiating innate immune responses.
  Related references:
  PMID:15361868
  PMID:18292575
  PMID:33718825
• Upon activation, MyD88 associates with IRAK1, IRAK2, IRF7, and TRAF6, triggering downstream NF-kappa-B activation and cytokine production.
  Related references:
  PMID:15361868
  PMID:19506249
  PMID:24316379
• MyD88 increases IL-8 transcription, contributing to pro-inflammatory responses.
  Related references:
  PMID:9013863
• MyD88 participates in the IL-18 signaling cascade and activates IRF1, promoting IFN-beta, NOS2, and IL12A expression for antiviral defense.
• Upon TLR8 activation by viral GU-rich RNA (e.g., from SARS-CoV-2, HIV-1), MyD88 drives NLRP3 inflammasome-mediated IL-1beta release.
  Related references:
  PMID:33718825
• MyD88 signaling in intestinal epithelial cells maintains gut homeostasis and controls antimicrobial lectin REG3G expression.
• MyD88 localizes to both the cytoplasm and nucleus, consistent with its signaling and regulatory roles.
• MyD88 is a phosphoprotein subject to ubiquitination, and alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blotting (WB), MyD88 migrates at an expected molecular weight of ~33 kDa. Include positive and negative controls, and consider using GAPDH or β-actin as loading controls.
• For immunofluorescence (IF/ICC), validate fixation and permeabilization protocols as MyD88 may be cytoplasmic, nuclear, or both depending on activation state. Co-staining with organelle markers can help confirm localization.
• For ELISA applications, peptide blocking using the immunogen can help verify specificity. Optimize coating and detection conditions for the target sample matrix.
• Since the immunogen (a.a. 20–140) covers the N-terminal region, this polyclonal antibody may detect multiple splice variants if epitopes are conserved. Check sequence alignment for the species and isoforms of interest.
• The reported cross-reactivity with mouse and rat suggests conserved epitopes; validate in the relevant model system with appropriate tissue or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a true one-stop platform for IVD raw-material procurement. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing support for rare targets—all tailored to meet rigorous diagnostic development demands. For innate immunity and inflammatory disease research, we help accelerate your assay development with reliable MyD88 antibodies and comprehensive supply-chain solutions.