Anti-MYBPC3 Polyclonal Antibody for WB, IHC-P, ELISA - Q14896

Core Product Specifications and Parameters

Biological Background: MYBPC3 Function and Localization

• MYBPC3 encodes the cardiac isoform of myosin-binding protein C, a thick filament-associated protein localized to the A bands of vertebrate striated muscle.
• It binds myosin heavy chain, F-actin, and native thin filaments, modulating actin-activated myosin ATPase activity and potentially playing both structural and regulatory roles in muscle contraction.
• The protein undergoes multiple post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination, which likely regulate its function and interactions.
• Structural features include immunoglobulin domains, zinc-binding sites, and disulfide bonds, contributing to its role in maintaining sarcomeric integrity.
• Alternative splicing generates different isoforms, and genetic variants are associated with cardiomyopathy, highlighting its critical role in cardiac muscle physiology.
• As a member of the myosin-binding protein family, it may also participate in cell adhesion processes.

Experimental Guidance and Technical Tips

• The immunogen targeting amino acids 200–400 may recognize conserved epitopes, consistent with observed cross-reactivity with mouse and rat. Validate specificity in your sample system.
• For Western blotting (WB), the predicted molecular weight is ~141 kDa; use appropriately low-percentage gels and optimized transfer protocols for high-molecular-weight proteins.
• In immunohistochemistry (IHC-P), consider testing different antigen retrieval methods and fixation conditions to ensure optimal signal.
• For ELISA applications, if using in a sandwich format, carefully select paired antibodies and validate assay linearity and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop platform for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support cardiac biomarker and cardiomyopathy research. From rare-target sourcing for MYBPC3-related assays to high-quality antibodies for muscle protein studies, CamelBio streamlines procurement from concept to clinic. Our integrated supply solutions bridge discovery and commercial manufacturing, ensuring efficiency and quality for diagnostic developers.