Anti-MYBPC2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q14324

Core Product Specifications and Parameters

Biological Background: Myosin-binding protein C, fast-type Function and Localization

• Myosin-binding protein C, fast-type (MYBPC2), also known as C-protein skeletal muscle fast isoform, is encoded by the MYBPC2 gene (GeneID: 4606). It is predominantly expressed as a thick filament-associated protein in vertebrate striated muscle.
• MYBPC2 localizes to the crossbridge region of the A bands in sarcomeres, where it interacts with myosin heavy chain (MHC), F-actin, and native thin filaments, thereby modulating actin-activated myosin ATPase activity.
• It plays a critical role in muscle contraction by regulating the kinetics of actomyosin interaction and contributing to the structural integrity of the sarcomere.
• The protein contains multiple immunoglobulin-like (Ig) domains and fibronectin type-III repeats, which mediate its binding to thick and thin filament proteins and are essential for its structural and regulatory functions.
• UniProt keywords highlight its involvement in actin binding, cell adhesion, and its classification as a muscle protein belonging to the immunoglobulin domain superfamily, underscoring its multifunctional nature in muscle biology.
• As the fast-type isoform of myosin-binding protein C, MYBPC2 is specifically expressed in fast-twitch skeletal muscle and is distinct from the cardiac (MYBPC3) and slow skeletal (MYBPC1) isoforms.

Experimental Guidance and Technical Tips

• When performing western blotting (WB), consider using a low-percentage SDS-PAGE gel (e.g., 6-8%) to achieve optimal resolution of the ~128kDa MYBPC2 protein. Reduced and denatured conditions are recommended.
• For immunohistochemistry (IHC-P), validate the antibody’s specificity using skeletal muscle tissue sections, where MYBPC2 is highly expressed in the A band of sarcomeres.
• In ELISA applications, the antibody may be used as a capture or detection reagent after appropriate pairing and optimization with other antibodies or antigens.
• Due to cross-reactivity with mouse and rat, this antibody is suitable for translational studies in these commonly used model organisms; however, target sequence homology should be verified for your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material sourcing, offering this anti-MYBPC2 polyclonal antibody alongside validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Whether you are advancing muscle-related disease diagnostics or basic muscle biology research, CamelBio provides tailored procurement solutions to streamline your development pipeline from concept to clinical application.