Anti-MYBPC2 Polyclonal Antibody for WB, ELISA - Q14324

Core Product Specifications and Parameters

Biological Background: MYBPC2 Function and Localization

• MYBPC2 (myosin-binding protein C, fast-type), also known as C-protein skeletal muscle fast isoform, is a key component of the thick filament in striated muscle.
• It localizes specifically to the crossbridge region of the sarcomeric A band, where it interacts with myosin heavy chain (MHC), F-actin, and native thin filaments.
• MYBPC2 modulates actin-activated myosin ATPase activity, suggesting a role in regulating muscle contraction; it may also serve a structural role within the sarcomere.
• The protein contains immunoglobulin-like domains and is implicated in cell adhesion processes, as indicated by its domain composition.
• MYBPC2 is primarily expressed in fast-type skeletal muscle fibers, consistent with its functional role in rapid contraction.
• 3D structural data are available for this protein, facilitating detailed molecular and functional studies.
• It belongs to the myosin-binding protein family and features repetitive immunoglobulin domains typical of muscle structural proteins.

Experimental Guidance and Technical Tips

• For Western blot assays, consider using reducing conditions and appropriate loading controls; the predicted molecular weight is approximately 128 kDa.
• Validate the antibody in your species of interest using positive control lysates (e.g., skeletal muscle tissues from human, mouse, or rat).
• As a polyclonal antibody raised against the C-terminal region (a.a. 912–1141), it may recognize multiple isoforms or degradation products; verify specificity in your experimental system.
• For ELISA applications, optimize coating antigen concentration and antibody titer; the immunogen sequence can serve as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For muscle biology and myosin-binding protein studies, CamelBio offers a range of validated antibodies and antigen pairs, including this MYBPC2 polyclonal antibody, to support your assay development and research. Our supply capabilities encompass bulk reagents, custom antibody generation, and sourcing of rare raw materials to streamline your workflow.