Anti-MYBBP1A Rabbit Polyclonal Antibody for WB - Q9BQG0

Core Product Specifications and Parameters

Biological Background: MYBBP1A Function and Localization

• MYBBP1A (Myb-binding protein 1A), also known as P160, PAP2, and Pol5, is a nuclear protein that can act as either a transcriptional activator or repressor depending on context.
• It functions as a corepressor, with repression potentially mediated through histone deacetylase (HDAC) activity.
• In the circadian clock, MYBBP1A cooperates with CRY1 to repress PER2 transcription by preferentially binding to dimethylated histone H3 'Lys-9' (H3K9me2) on the PER2 promoter.
• MYBBP1A plays a role in rRNA biogenesis in partnership with PWP1, as evidenced by studies showing its involvement in pre-rRNA processing. Related references: PMID:29065309
• Localization studies indicate MYBBP1A is found in the cytoplasm, nucleus, and prominently in the nucleolus, consistent with its dual roles in transcriptional regulation and ribosome biogenesis.
• The protein is subject to multiple post-translational modifications, including acetylation, citrullination, phosphorylation, and ubiquitin-like conjugation (ISGylation), which may modulate its activity.
• Additional keywords highlight its involvement in biological rhythms, alternative splicing, and proteomic identification, underscoring its dynamic regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal fragment (aa 1104-1328) of human MYBBP1A; the antibody may recognize full-length protein and potentially isoforms—validate in your specific sample system.
• This antibody is suitable for WB, IHC-P, IF/ICC, and ELISA. For WB, expect a band near 149 kDa; for immunostaining, anticipate nuclear/nucleolar localization. Optimize dilutions and antigen retrieval conditions experimentally.
• It cross-reacts with human, mouse, and rat samples; however, cross-reactivity in other species should be verified if needed.
• Consider the protein's multiple modifications and dynamic localization when designing experiments, and include appropriate controls for detection specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your diagnostic development by offering thoroughly characterized antibody raw materials like this anti-MYBBP1A polyclonal antibody, suitable for transcription and ribosome biogenesis research. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets, ensuring a seamless supply chain from concept to clinic.