Anti-MXI1 Rabbit Polyclonal Antibody for WB, ELISA - P50539

Core Product Specifications and Parameters

Biological Background: MXI1 Function and Localization

• MXI1 (Max-interacting protein 1) is a transcriptional repressor encoded by the MXI1 gene, also designated as Class C basic helix-loop-helix protein 11 (bHLHc11) (UniProt P50539).
• It functions by heterodimerizing with MAX to form a sequence-specific DNA-binding complex that recognizes the E-box consensus 5'-CAC[GA]TG-3', thereby antagonizing MYC-driven transcriptional activation through competition for MAX.
• As a member of the Myc/Max/Mad network, MXI1 exhibits alternative splicing and is classified as a proto-oncogene, with disease-associated variants implicated in cancer susceptibility.
• Subcellular localization is predominantly nuclear, consistent with its role in transcription regulation and DNA-binding.
• Tissue expression is enriched in brain, heart, and lung, with comparatively lower levels in liver, kidney, and skeletal muscle.
• The protein has a calculated molecular weight of approximately 26 kDa (consistent with the antibody’s detected weight of 26 kDa), and contains basic helix-loop-helix and leucine zipper motifs essential for protein–protein and protein–DNA interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 76–295 of human MXI1, which encompasses the functional bHLH and leucine zipper regions critical for MAX binding and DNA recognition; this design may favor detection of both full-length and relevant isoforms.
• For Western blotting, consider starting with a dilution range of 1:500–1:2,000 and validate the antibody using lysates from cell lines or tissues with known MXI1 expression (e.g., brain, heart, lung).
• In ELISA applications, direct coating with the recombinant immunogen or peptide may be employed; optimal coating conditions and detection antibody concentrations should be determined empirically.
• Cross-reactivity with mouse and rat homologs is reported, allowing the antibody to be used in orthologous model systems, but always verify species-specific reactivity in your experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research institutions with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. For researchers focused on transcriptional regulation and MYC pathway antagonism, CamelBio can efficiently source MXI1-related products and scale up supply to meet project demands, from early-stage development to clinical validation.