Anti-MXI1 Rabbit Polyclonal Antibody for WB and ELISA - P50539

Core Product Specifications and Parameters

Biological Background: MXI1 Function and Localization

• MXI1 (Max-interacting protein 1), also designated MXD2 and bHLHc11, belongs to the basic helix-loop-helix (bHLH) family of transcription factors.
• It acts as a transcriptional repressor by heterodimerizing with MAX and binding to the E-box consensus sequence 5'-CACGTG-3', thereby competing with MYC for MAX and antagonizing MYC-mediated transcriptional activation.
• As a proto-oncogene, genetic alterations in MXI1 are linked to human cancers and other diseases (keyword: Disease variant).
• The protein localizes predominantly to the nucleus, consistent with its role in DNA binding and transcriptional regulation.
• Tissue expression is broad: highest levels occur in brain, heart, and lung, with lower levels in liver, kidney, and skeletal muscle.
• Multiple isoforms are generated through alternative splicing, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• For Western blot, anticipate a specific band near 26 kDa; verify expression and molecular weight in your sample system.
• The antibody is documented to cross-react with human, mouse, and rat MXI1; confirm reactivity in your species of interest with appropriate controls.
• Optimize antibody dilution and incubation parameters for ELISA; titrate to achieve optimal signal-to-noise ratio.
• Because this is a polyclonal antibody, lot-to-lot consistency should be validated in critical or quantitative experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our anti-MXI1 polyclonal antibody is well-suited for transcription regulation and oncology research, and we can assist with sourcing rare targets or scaling up your material needs. Connect with our team to explore custom sourcing solutions and technical support—from concept to clinic.