Anti-MVB12A Polyclonal Antibody for WB, ELISA - Q96EY5

Core Product Specifications and Parameters

Biological Background: MVB12A Function and Localization

• Multivesicular body subunit 12A (MVB12A) is a component of the ESCRT-I complex, a key regulator of vesicular trafficking that sorts ubiquitinated cargos into multivesicular bodies (MVBs).
• MVB12A is required for the endocytic sorting of ubiquitinated membrane proteins, directing them into the lumen of MVBs and preventing recycling to the plasma membrane.
• It plays a specific role in the ligand-mediated internalization and down-regulation of the epidermal growth factor receptor (EGFR), promoting its lysosomal degradation.
• MVB12A is localized to multiple cellular compartments, including the cytoplasm, nucleus, endosomes, centrosomes, and the membrane of late endosomes, reflecting its dynamic trafficking functions.
• The protein is ubiquitously expressed in human tissues, with the notable exception of skeletal muscle, suggesting broad but not universal roles in membrane protein sorting.
• MVB12A is a phosphoprotein and contains an SH3-binding motif, enabling interactions with CIN85/CD2AP family adaptors and linking it to actin cytoskeleton dynamics.
• Alternative splicing generates multiple isoforms, which may contribute to functional diversity in ESCRT-I complex assembly and cargo selection.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-273 of human MVB12A (full-length), suitable for detecting total MVB12A protein.
• For Western blotting, the predicted molecular weight is approximately 29 kDa; a band near this size should be observed in relevant samples.
• The antibody is reported to cross-react with rat, making it useful for studies in rodent models; however, validation in the specific experimental system is recommended.
• In ELISA, working dilutions should be optimized by the end user; consider using the recombinant immunogen as a positive control.
• Endogenous expression is ubiquitous except in skeletal muscle; whole-cell lysates from common cell lines (e.g., HeLa, HEK293) are suitable for initial testing.
• Given the involvement of MVB12A in EGFR down-regulation, stimulation with EGF may alter protein levels or localization, providing a functional validation approach.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes integrated access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This MVB12A polyclonal antibody supports research in vesicular trafficking and receptor down-regulation pathways, and CamelBio can assist with complementary reagents (e.g., recombinant proteins, detection antibodies) for your endocytic trafficking assays. Connect with us to streamline your supply chain from concept to clinic.