Anti-MUTYH Polyclonal Antibody for IHC-P, IF/ICC, ELISA - Q9UIF7

Core Product Specifications and Parameters

Biological Background: MUTYH Function and Localization

• MUTYH (MutY homolog, also known as hMYH) is an adenine DNA glycosylase involved in base excision repair of oxidative DNA damage.
• It initiates repair of A:8-oxoguanine (A*oxoG) mismatches by excising the mispaired adenine, promoting restoration to C:G base pairs.
• The enzyme possesses both adenine and 2-hydroxyadenine (2-OH-A) DNA glycosylase activities.
• MUTYH localizes to both the nucleus and mitochondria, consistent with its role in repairing nuclear and mitochondrial genomes.
• It contains a 4Fe-4S iron-sulfur cluster and binds metal ions, which are important for its structural integrity and enzymatic function.
• Multiple isoforms are produced by alternative splicing; mutations in MUTYH are associated with MUTYH-associated polyposis (MAP) and increased colorectal cancer risk, classifying it as a tumor suppressor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-265) of human MUTYH, which may yield antibodies recognizing multiple isoforms; consider validating with relevant control lysates.
• The antibody is suited for IHC-P, IF/ICC, and ELISA applications according to the supplier; optimal dilutions should be empirically determined for each assay.
• Cross-reactivity with human and rat has been specified; verify performance in other species if required.
• For IHC-P, appropriate antigen retrieval and blocking steps are recommended; for IF/ICC, include mitochondrial and nuclear counterstains to confirm localization.
• ELISA may be used for detection of recombinant or endogenous MUTYH; ensure the antibody pair is validated for specificity and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material partner, CamelBio supports diagnostic developers and researchers with reliable antibodies, proteins, and technical resources for DNA repair and oncology targets like MUTYH. From validated antibody pairs to bulk ancillary reagents, we streamline sourcing to help you move from discovery to diagnostic application. Contact us to discuss your custom antibody or raw-material needs.