Anti-Muscarinic AChR M2 (ACM2) Rabbit Polyclonal Antibody for WB, ELISA - P08172

Core Product Specifications and Parameters

Biological Background: Muscarinic Acetylcholine Receptor M2 Function and Localization

• CHRM2 encodes the muscarinic acetylcholine receptor M2 (ACM2), a neurotransmitter receptor that binds acetylcholine and transduces signals across the cell membrane. Related references: PMID:24256733 PMID:3443095 PMID:36690613
• Ligand binding causes a conformational change that triggers signaling through G(i)/G(o) G proteins (GNAI1 or GNAO1), leading to inhibition of adenylate cyclase activity. Related references: PMID:36690613
• The receptor localizes predominantly to the cell membrane, with a specific presence at the postsynaptic cell membrane, underscoring its role in synaptic transmission.
• As a member of the G protein-coupled receptor (GPCR) superfamily, ACM2 contains seven transmembrane helices, disulfide bonds, and glycosylation sites, all critical for its structural and functional integrity.
• Key post-translational modifications and annotations include phosphorylation and a 3D-structure resolved by experimental methods, classifying it as a transducer with reference proteome status.

Experimental Guidance and Technical Tips

• The immunogen (aa 208–388) spans intracellular loop regions and portions of transmembrane domains, making the antibody suitable for detecting denatured receptor in Western blotting; consider reducing conditions for optimal exposure of epitopes.
• For ELISA applications, the recombinant immunogen can serve as a positive control to validate coating and detection conditions.
• Validate antibody specificity in CHRM2-expressing cell lines or tissues (e.g., brain, heart) and confirm molecular weight near the predicted 52 kDa band under your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers and research institutions by offering validated antibodies, optimized pairs, and bulk ancillary reagents. This anti-Muscarinic AChR M2 antibody supports neuroscience and cardiovascular assay development, with the capability to source rare targets and provide technical consulting from concept to clinic.