Anti-Muscarinic AChR M2 (ACM2) Monoclonal Antibody for WB, IHC-P, ELISA - P08172

Core Product Specifications and Parameters

Biological Background: Muscarinic AChR M2 Function and Localization

• Muscarinic acetylcholine receptor M2 (encoded by the CHRM2 gene) is a G protein-coupled receptor that binds the neurotransmitter acetylcholine, mediating cellular responses in the brain, heart, and autonomic nervous system.
• Ligand binding triggers a conformational change and activates intracellular signaling via guanine nucleotide-binding proteins (G proteins).
• CHRM2 preferentially couples to G(i)/G(o) proteins (GNAI1 or GNAO1), leading to inhibition of adenylate cyclase activity and reduction of cAMP levels. Related references: PMID:24256733, PMID:3443095, PMID:36690613
• The receptor is located at the cell membrane, specifically enriched at postsynaptic membranes in cholinergic synapses.
• Structurally, M2 is a glycoprotein with seven transmembrane helices, characteristic of class A GPCRs, and undergoes post-translational modifications including phosphorylation and disulfide bond formation.
• Key biological roles include modulation of heart rate, smooth muscle contraction, and regulation of neurotransmitter release in the central nervous system.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100–200 of human M2, a region that may correspond to the second extracellular loop or adjacent transmembrane domains; consider this when predicting epitope accessibility in native versus denaturing conditions.
• For Western blotting, the predicted molecular weight is approximately 52 kDa; validate band specificity using positive and negative cell lysates or peptide competition assays.
• In immunohistochemistry (paraffin-embedded sections), M2 expression is expected in cholinergically innervated tissues such as the heart, brain, and smooth muscle; optimize antigen retrieval and validate staining patterns against known expression profiles.
• For ELISA, the antibody may be used as a capture or detection reagent; pair with a suitable coating antigen and confirm cross-reactivity against M2 from mouse and rat if required.

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CamelBio connects diagnostic manufacturers and research labs with a complete spectrum of IVD raw materials, including this anti-M2 muscarinic receptor antibody for GPCR and neuroscience applications. Our portfolio covers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—streamlining your development from early feasibility to clinical deployment. Reach out to explore how our tailored supply solutions can support your next project.