Anti-Musashi-1 (MSI1) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O43347

Core Product Specifications and Parameters

Biological Background: Musashi-1 (MSI1) Function and Localization

• Musashi-1 (MSI1) is an RNA-binding protein belonging to the Musashi family, which plays a crucial role in post-transcriptional gene regulation.
• It specifically binds to RNA sequences containing the motif 5'-GUUAGUUAGUUAGUU-3' and other sequences with the pattern 5'-[GA]U(1-3)AGU-3', regulating target mRNA translation.
• MSI1 is a key regulator of stem cell maintenance and proliferation, particularly in the central nervous system, by controlling the expression of the NOTCH1 antagonist NUMB.
• It is expressed in various tissues, including fetal kidney, brain, liver, and lung, and in adult brain and pancreas, with detection in hepatoma cell lines.
• Subcellularly, MSI1 localizes to both the cytoplasm and nucleus.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 200-362 of human MSI1, which should provide specific recognition of the full-length protein.
• For Western blotting (WB), a band at approximately 39 kDa is expected; consider using appropriate positive controls such as brain or stem cell lysates.
• In immunohistochemistry (IHC-P), validate antibody specificity in tissues known to express MSI1, such as brain or fetal tissues; antigen retrieval may be required.
• For ELISA, this antibody can be used for detection of recombinant or native MSI1; optimize coating and detection conditions empirically.
• The antibody is reported to cross-react with mouse and rat MSI1, but sequence homology should be confirmed if working with these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner, providing diagnostic manufacturers, research institutes, and laboratories with high-quality IVD raw materials, technical services, and expert consulting from concept to clinic. This anti-MSMSI1 rabbit polyclonal antibody is ideal for researchers studying stem cell biology, neurodevelopment, and cancer. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored rare-target sourcing to accelerate your assay development and diagnostic applications.