Anti-Musashi-1 (MSI1) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - O43347

Core Product Specifications and Parameters

Biological Background: Musashi-1 (MSI1) Function and Localization

• MSI1 (RNA-binding protein Musashi homolog 1) is an RNA-binding protein that primarily regulates target mRNA translation.
• It binds specific RNA motifs, such as 5'-GUUAGUUAGUUAGUU-3' and the pattern 5'-[GA]U(1-3)AGU-3', to control gene expression post-transcriptionally.
• MSI1 modulates the expression of NUMB, a NOTCH1 antagonist, implicating it in Notch signaling pathway regulation.
• The protein localizes to both the cytoplasm and nucleus, consistent with its role in mRNA transport and translational control.
• MSI1 is expressed in fetal tissues (kidney, brain, liver, lung) and in adult brain and pancreas; it is also detected in hepatoma cell lines, suggesting roles in development and cancer.
• It is phosphorylated and acetylated, which may regulate its activity, RNA-binding affinity, or subcellular distribution.
• MSI1 contains RNA recognition motifs (RRMs) and belongs to the Musashi family of RNA-binding proteins, with a molecular weight of approximately 39 kDa.
• Key biological processes include stem cell proliferation and maintenance in the central nervous system, as well as regulation of neural differentiation.

Experimental Guidance and Technical Tips

• Design experiments using the N-terminal immunogen (aa 1-100) to ensure recognition of all known isoforms; validate in relevant sample types such as brain or cancer cell lines.
• For Western blot, the expected band is approximately 39 kDa; include positive controls like human or mouse brain lysate.
• In immunofluorescence/ICC, check for both cytoplasmic and nuclear staining; permeabilization may be required.
• For ELISA applications, consider using the antibody as a capture or detection reagent with appropriate blocking and washing steps.
• Since it is a rabbit monoclonal, lot-to-lot reproducibility is high; however, independent validation in your system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers working on stem cell biology or RNA-binding proteins, this anti-MSI1 antibody is part of our extensive catalog of validated monoclonal and polyclonal antibodies. We also offer bulk reagent sourcing, antibody pair development, and customized solutions to streamline your project from concept to clinic.