Anti-MUS81 Polyclonal Antibody for WB, IHC-P, ELISA - Q96NY9

Core Product Specifications and Parameters

Biological Background: Structure-specific endonuclease subunit MUS81 Function and Localization

• MUS81 is the catalytic subunit of two structure-specific heterodimeric DNA endonucleases, MUS81-EME1 and MUS81-EME2, which play critical roles in maintaining genome stability. Related references: PMID:11741546 PMID:12374758 PMID:12686547
• Both MUS81-EME1 and MUS81-EME2 cleave 3'-flaps and nicked Holliday junctions, with limited activity on 5' flaps and nicked double-stranded DNA; MUS81-EME2 exhibits higher catalytic activity. Related references: PMID:24371268 PMID:24733841 PMID:35290797
• MUS81-EME2 functions during DNA replication by processing stalled replication forks, generating double-strand breaks that facilitate fork restart via homologous recombination; it may also be involved in telomere maintenance. Related references: PMID:24813886
• MUS81-EME1 acts later in the cell cycle to resolve mitotic recombination intermediates, including Holliday junctions, thereby ensuring proper chromosome segregation. Related references: PMID:11741546 PMID:12374758 PMID:14617801
• MUS81 localizes predominantly to the nucleus and nucleolus, consistent with its roles in DNA repair and recombination.
• The protein is subject to phosphorylation, as indicated by proteomics data, though the functional significance of specific modifications remains to be fully elucidated.
• MUS81 is widely expressed across human tissues, reflecting its fundamental role in genome maintenance.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-350) of human MUS81; consider this when designing detection strategies for isoforms or truncated forms.
• For WB, a band around 61 kDa is expected; validate specificity in appropriate positive and negative control lysates.
• When performing IHC-P, optimize antigen retrieval and antibody dilution using known positive tissues (e.g., human tonsil or testis).
• For ELISA, the antibody may be used for capture or detection; pairing with a matched antibody may improve assay sensitivity.
• Cross-reactivity with mouse and rat has been noted, but validate in the relevant model system to ensure specificity.
• Use non-ionic detergents in lysis buffers to maintain MUS81 solubility and native conformation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw-material sourcing, supporting diagnostic developers and research teams in accessing high-quality reagents like this MUS81 polyclonal antibody for DNA repair and genome stability studies. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development. Let CamelBio bridge the gap from concept to clinic with reliable, one-stop access to the critical materials you need.