Anti-Munc 13-1 Rabbit Polyclonal Antibody for WB, ELISA - Q9UPW8

Core Product Specifications and Parameters

Biological Background: Munc 13-1 Function and Localization

• Protein unc-13 homolog A (UNC13A, also known as Munc13-1) is a 193 kDa multi-domain protein containing C2 domains, phorbol ester/diacylglycerol-binding zinc fingers, and a C-terminal MUN domain essential for synaptic vesicle priming.
• It functions as a priming factor in synaptic vesicle exocytosis, essential for most excitatory/glutamatergic synapses, and participates in activity-dependent refilling of the readily releasable vesicle pool (RRP) (By similarity).
• Acts downstream of diacylglycerol second messenger signaling to mature vesicles for fusion, linking intracellular calcium signals to regulated neurotransmitter and hormone secretion.
• Expressed in pancreatic islet cells, Munc13-1 primes insulin secretory granules for release, contributing to glucose-stimulated insulin secretion.
  Related references:
  PMID:12871971
• Plays a role in dendrite formation by melanocytes.
  Related references:
  PMID:23999003
• Localizes to the presynaptic active zone, cytoplasm, and cell membrane, where it positions synaptic vesicles near calcium channels for rapid exocytosis.
• Contains metal-binding domains (calcium, zinc) and phorbol-ester/DAG-type zinc fingers, reflecting its regulation by second messengers and metal ions.
• Mutations in UNC13A are implicated in autosomal dominant epilepsy and intellectual disability, underscoring its critical role in synaptic transmission and neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen is recombinant human Munc13-1; for species not listed under Cross Reactivity, sequence homology should be checked to predict potential cross-reactivity.
• For Western blot, expect a band near 193 kDa; use low-percentage gels and extended transfer times for high molecular weight proteins, and consider reducing conditions.
• For ELISA, validate detection using recombinant protein or lysates from tissues expressing Munc13-1 (e.g., brain, pancreas).
• Include appropriate negative controls (e.g., knockout cell lysates) to confirm antibody specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, including validated polyclonal antibodies like this anti-Munc13-1 product. For neuroscience and metabolic disease assay development, our platform enables streamlined procurement of antibody pairs, recombinant proteins, and ancillary reagents, with dedicated support for rare-target material acquisition.