Anti-MUM1 Rabbit Monoclonal Antibody for IF-P, IHC-P, ELISA - Q15306

Core Product Specifications and Parameters

Biological Background: IRF4/MUM1 Function and Localization

• IRF4 (Interferon regulatory factor 4), also known as MUM1, LSIRF, SHEP8, and NF-EM5, is a member of the interferon regulatory factor (IRF) family of transcription factors.
• Functions as a transcriptional activator, binding to the interferon-stimulated response element (ISRE) of the MHC class I promoter.
• Physically interacts with PU.1 to bind the immunoglobulin lambda light chain enhancer, playing a role in lymphoid-specific signal transduction.
• In immune cells, IRF4 forms a complex with BATF-JUNB heterodimer to drive CD8+ dendritic cell differentiation via recognition of the AICE sequence (5'-TGAnTCA/GAAA-3').
• Localizes to both the nucleus and cytoplasm; predominantly nuclear upon activation.
• Expressed specifically in lymphoid cells, underscoring its role in lymphocyte development and immune regulation.
• Post-translational modifications include phosphorylation; the protein is also subject to alternative splicing and chromosomal rearrangement (e.g., in multiple myeloma).
• Keywords: transcriptional activator, DNA-binding, chromosomal rearrangement, disease variant, alternative splicing, phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 351-451) of human IRF4; the antibody may recognize isoforms containing this region, but users should verify cross-reactivity with alternative splice variants.
• For immunofluorescence (IF-P) and immunohistochemistry (IHC-P), optimize fixation and permeabilization protocols, as IRF4 can localize to both nucleus and cytoplasm; include positive control tissues such as lymphoid tissue or multiple myeloma samples.
• In ELISA, the antibody can be used with recombinant IRF4 protein or cell lysates; consider using a blocking buffer compatible with rabbit monoclonal antibodies.
• For chromatin immunoprecipitation (ChIP), the antibody may be suitable given IRF4's DNA-binding properties, but requires independent validation.
• Verify specificity by performing knockdown or knockout controls in human lymphoid cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibodies, bulk ancillary reagents, and custom development services. For researchers investigating lymphoid transcription factors and immune regulation, this high-quality anti-MUM1 rabbit monoclonal antibody is available with consistent lot-to-lot performance. Whether you need paired antibodies, large-scale production, or rare target sourcing, CamelBio provides end-to-end support from concept to clinic.