Anti-Multimerin-1 (MMRN1) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13201

Core Product Specifications and Parameters

Biological Background: Multimerin-1 Function and Localization

• Multimerin-1 (MMRN1), also known as EMILIN-4 or elastin microfibril interfacer 4, is a secreted glycoprotein belonging to the EMILIN family of extracellular matrix proteins.
• It functions as a carrier protein for platelet factor V/Va, playing a critical role in the storage and stabilization of factor V within platelet alpha granules.
• Upon platelet activation, MMRN1 is released and may limit factor Va-dependent thrombin generation, thereby regulating coagulation.
• MMRN1 serves as a ligand for integrin alpha-IIb/beta-3 and integrin alpha-V/beta-3 on activated platelets, suggesting a role as an adhesive protein in platelet plug formation and thrombus stabilization.
• Subcellularly, MMRN1 is secreted and stored in platelet alpha granules and endothelial cell Weibel-Palade bodies, from which it is released upon cellular activation and deposited onto the endothelium and subendothelium.
• Synthesized primarily by endothelial cells and megakaryocytes, MMRN1 is found in vascular tissues such as placenta, lung, and liver, but is not detected in normal plasma.
• The protein contains an N-terminal signal peptide, coiled-coil domains, EGF-like domains, and disulfide bonds, which contribute to its structural stability and multimerization.

Experimental Guidance and Technical Tips

• Since the immunogen corresponds to the C-terminal region (amino acids 1083-1228), this antibody is expected to recognize full-length MMRN1 as well as any isoforms containing this region. For Western blot, a band around 138 kDa is anticipated; validate using platelet or endothelial cell lysates.
• For immunofluorescence/immunocytochemistry (IF/ICC), permeabilization may be required to detect intracellular stores in platelet alpha granules or Weibel-Palade bodies; consider co-staining with markers like von Willebrand factor for endothelial cells.
• In ELISA applications, this polyclonal antibody can be used as a capture or detection reagent; optimize coating and detection conditions using recombinant MMRN1 protein or platelet releasate.
• Given cross-reactivity with mouse, this antibody is suitable for studies in murine models of thrombosis and vascular injury; validate specificity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with convenient, one-stop access to IVD raw materials. For MMRN1 research and thrombosis-related diagnostic development, we can supply this high-quality rabbit polyclonal antibody along with validated antibody pairs, optimized monoclonal or polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, supporting your projects from concept to clinic efficiently.