Anti-Mucin 5AC Rabbit Polyclonal Antibody for WB and ELISA - P98088

Core Product Specifications and Parameters

Biological Background: Mucin 5AC Function and Localization

• Mucin 5AC is a large secreted gel-forming glycoprotein that constitutes the major macromolecular component of mucus in the respiratory and gastric tracts, providing a protective barrier against infection and chemical damage.

• It binds to inhaled microorganisms and particles, which are then cleared from the respiratory tract via the mucociliary escalator, playing a critical role in innate defense. Related references: PMID:14535999 PMID:14718370

• Mucin 5AC interacts with Helicobacter pylori, facilitating bacterial adherence in gastric epithelium, Barrett's esophagus, and duodenal gastric metaplasia, thereby contributing to H. pylori-associated pathologies. Related references: PMID:14535999

• Expression is normally restricted to surface mucosal cells of the respiratory tract and stomach, but it is frequently overexpressed in carcinomas, including lung, gastric, and colorectal cancers, where it may serve as a biomarker.

• As a secreted protein bearing extensive O-linked glycosylation and numerous disulfide bonds, mucin 5AC forms high-molecular-weight polymers that confer viscoelastic properties to mucus. Its structure contains characteristic cysteine-rich D domains and central mucin-type repeats.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the central region (aa 1550–1900) of human Mucin 5AC. This region may be glycosylated in the native protein; verify that the antibody recognizes the mature glycoprotein under your experimental conditions.
• For Western blot, consider using reducing agents to break disulfide bonds, as the native mucin forms very large multimers (>500 kDa). Migration may be affected by glycosylation, so use appropriate molecular weight markers and optimize transfer conditions.
• This polyclonal antibody cross-reacts with mouse and rat Mucin 5AC, making it suitable for translational studies in rodent models. Validate species reactivity in your specific tissue lysates.
• ELISA applications may require coating conditions (e.g., carbonate buffer, pH 9.6) and blocking steps optimized for large glycoproteins. Include a negative control such as a non-mucin protein.
• As with any polyclonal antibody, lot-to-lot variability can occur. Consider performing a bridging study when switching lots and confirm performance in your established assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. For mucin-related research and diagnostic development, we offer validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary supplies, and bespoke sourcing for rare target materials. Our integrated supply solutions help you maintain consistent assay performance and accelerate your path to market.