Anti-MUC4 Rabbit Polyclonal Antibody for WB, ELISA - Q99102

Core Product Specifications and Parameters

Biological Background: Mucin-4 Function and Localization

• Mucin-4 (MUC4) is a membrane-bound, highly glycosylated mucin that constitutes the major component of mucus covering epithelial surfaces. Related references: PMID:10880978
• It plays critical roles in epithelial protection, renewal, and differentiation. Related references: PMID:10880978
• MUC4 regulates cellular behavior through anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and serves as an intramembrane ligand for ERBB2, inducing specific ERBB2 phosphorylation to promote proliferation and differentiation of epithelial cells.
• Interaction with ERBB2 enhances expression of CDKN1B; formation of a MUC4-ERBB2-ERBB3-NRG1 complex down-regulates CDKN1B, leading to repression of apoptosis and stimulation of proliferation, which may contribute to tumor growth.
• Alternative splicing yields membrane-bound and secreted isoforms; the protein localizes to the cell membrane and is also secreted.
• It is expressed in a wide range of epithelial tissues (thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, mammary and salivary glands) and in carcinomas of lung, upper aerodigestive tract, breast, biliary tract, colon, and cervix; minimal expression in normal pancreas but high expression in pancreatic tumors.
• Key structural features include an EGF-like domain, disulfide bonds, and a transmembrane helix; classified under alternative splicing, glycoprotein, cell adhesion, membrane, and secreted.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 2100-2169 of human MUC4; this sequence may be used for designing custom assay controls or blocking experiments.
• For Western blot, note the large predicted molecular weight of ~542 kDa; low-percentage SDS-PAGE gels and extended transfer times are recommended for efficient detection.
• For ELISA, peptide-based competitive or sandwich formats may be considered; validation in relevant sample systems (e.g., cell lysates, tissue extracts) is advised.
• Due to high glycosylation and alternative splicing, multiple bands or isoforms may appear; consult UniProt record Q99102 for isoform details.

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CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-MUC4 polyclonal antibody supports oncology and epithelial biology research. Discover our extensive portfolio, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare target raw materials.