Anti-MUC4 Monoclonal Antibody for WB, IHC-P, ELISA - Q99102

Core Product Specifications and Parameters

Biological Background: MUC4 Function and Localization

• MUC4 is a high-molecular-weight (542 kDa) transmembrane glycoprotein belonging to the mucin family, characterized by extensive O-glycosylation and a large extracellular domain with EGF-like repeats.
• It serves as a major component of the mucus layer, providing a protective barrier on epithelial surfaces and contributing to epithelial cell renewal and differentiation. Related references: PMID:10880978
• MUC4 functions as an intramembrane ligand for the receptor tyrosine kinase ERBB2, inducing ERBB2 phosphorylation and modulating downstream signaling pathways to influence cell proliferation, differentiation, and apoptosis; it can form complexes with ERBB3 and NRG1 that repress apoptosis and promote tumor growth. Related references: PMID:10880978
• The protein undergoes alternative splicing and contains disulfide bonds, EGF-like domains, and a transmembrane helix; it is annotated with keywords such as glycoprotein, cell adhesion, and secreted (referring to its shed ectodomain).
• Subcellular localization includes the cell membrane as a type I membrane protein, but the ectodomain can be cleaved and secreted; it is found in the membrane and secreted fractions.
• Tissue expression is broad: normal expression in thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, mammary and salivary glands; it is overexpressed in multiple carcinomas, including pancreatic, lung, breast, biliary, colon, and cervical cancers, while being minimal in normal pancreas.
• Given its role in cell adhesion modulation and ERBB2 interaction, MUC4 is implicated in epithelial-mesenchymal transition and cancer progression, making it a valuable marker in oncology research.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the C-terminal part of MUC4 (amino acids 4807-4916), which is part of the extracellular domain. This region may be less glycosylated than the tandem repeat region, potentially offering higher specificity in detecting the core protein.
• Given the large size of MUC4 (542 kDa), consider using low percentage SDS-PAGE (e.g., 4-6%) for western blot to enhance transfer efficiency. Extended transfer times and low voltage may improve detection.
• For IHC-P, antigen retrieval methods (such as heat-induced epitope retrieval in citrate buffer, pH 6.0) may be necessary; validation in known positive and negative tissue controls (e.g., pancreatic tumor vs. normal pancreas) is recommended.
• Cross-reactivity with rat has been reported; validation in rat samples should be performed to confirm suitability for rodent studies.
• Due to the high molecular weight and heavy glycosylation, lysate preparation may require sonication and use of denaturing conditions to ensure solubility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers integrated IVD raw-material solutions for diagnostic manufacturers and researchers. Our portfolio features this rabbit monoclonal anti-MUC4 antibody to support oncology and epithelial biology studies, complemented by validated antibody pairs, bulk ancillary reagents, and sourcing for rare mucin targets. From early-stage research to clinical application, CamelBio streamlines your supply chain.