Anti-MUC20 Rabbit Polyclonal Antibody for WB and ELISA - Q8N307

Core Product Specifications and Parameters

Biological Background: MUC20 Function and Localization

• MUC20 encodes Mucin-20, a secreted and membrane-associated glycoprotein involved in regulating MET signaling.
• It decreases hepatocyte growth factor (HGF)-induced transient MAPK activation by blocking GRB2 recruitment to MET, suppressing the GRB2-RAS pathway.
• MUC20 inhibits HGF-induced proliferation and expression of MMP1 and MMP9.
• The protein is localized to the apical and basolateral cell membrane, microvillus membrane, and is also secreted.
• MUC20 shows high expression in kidney (proximal tubules), moderate in placenta, lung, prostate, liver, and digestive system; it is detected in most male urogenital tract epithelia except epididymis.
• Alternative splicing generates multiple isoforms, and the protein contains repeat regions and a signal peptide (UniProt Keywords: Alternative splicing, Glycoprotein, Repeat, Signal).
• The molecular weight is approximately 72 kDa (UniProt: 71982 Da).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the central region (aa 100-200) of human MUC20, suggesting the antibody may recognize both secreted and membrane-bound forms. Consider using appropriate positive controls (e.g., kidney or prostate tissue lysates) when validating for WB.
• For ELISA, the antibody may be suitable for detecting MUC20 in cell supernatants or tissue extracts. Optimize coating and detection conditions for your specific assay format.
• Given cross-reactivity with mouse and rat, this antibody can be considered for orthologous studies; however, validate specificity and sensitivity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing platform for IVD raw materials, providing diagnostic manufacturers and research teams with high-quality antibodies like this MUC20 polyclonal antibody. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing support for rare targets—streamlining your workflow from concept to clinic. For researchers focused on MET signaling and urogenital disease, CamelBio ensures reliable access to critical immunoassay components tailored to your needs.