Anti-MUC2 Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q02817

Core Product Specifications and Parameters

Biological Background: MUC2 Function and Localization

• Mucin-2 (MUC2) is a large secreted glycoprotein, also known as intestinal mucin-2, encoded by the MUC2 gene.
• It serves as a major component of intestinal mucus, coating the epithelium to provide a protective and lubricating barrier against particles, pathogens, and infectious agents. Related references: PMID:17058067, PMID:19432394, PMID:33031746
• In the colon, MUC2 forms large polymeric networks that create hydrogels, safeguarding the epithelium while allowing nutrient absorption and gas exchange. Related references: PMID:33031746, PMID:36206754
• MUC2 functions as a divalent copper chaperone, binding both Cu2+ and Cu1+ to protect intestinal cells from copper toxicity and facilitate nutritional copper uptake. Related references: PMID:36206754
• The protein undergoes O-glycosylation, autocatalytic cleavage, and disulfide bond formation, which are critical for its gel-forming properties and stability.
• MUC2 is highly expressed in the colon, small intestine, and also found in colonic tumors, bronchus, cervix, and gall bladder.
• The mucin gel stores antimicrobial molecules, contributing to innate immune defense at mucosal surfaces. Related references: PMID:33031746
• Goblet cells produce two forms of MUC2 differing in O-glycosylation: a 'thick' mucus in the proximal colon that encapsulates microbiota, and a 'thin' lubricating mucus in the distal colon.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 850–1270 of human MUC2 (NP_002448.4); consider this region for epitope mapping or orthogonal validation.
• For Western blot analysis, use low-percentage SDS-PAGE gels (e.g., 4–6%) and extended transfer times to accommodate the ~551 kDa protein; whole-cell lysates from colon-derived cell lines (e.g., Caco-2) or intestinal tissue are suitable samples.
• In IHC-P, formalin-fixed paraffin-embedded colon sections are recommended; antigen retrieval (e.g., citrate buffer, pH 6.0) may improve signal.
• For IF-P, optimize permeabilization and blocking conditions; co-staining with cell-specific markers can confirm localization in goblet cells.
• The antibody can be used in ELISA for MUC2 detection; pairing with a monoclonal MUC2 antibody in a sandwich format may enhance specificity and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target procurement to support MUC2-related diagnostic and research applications. Whether you require large-scale production of MUC2 antibodies or complementary reagents for mucosal barrier studies, our end-to-end supply chain ensures seamless delivery. Contact our team to discuss custom sourcing solutions for your next project.