Anti-MUC16 (CA125) Polyclonal Antibody for WB and ELISA - Q8WXI7

Core Product Specifications and Parameters

Biological Background: Mucin-16 (MUC16/CA125) Function and Localization

• Mucin-16 (MUC16), also designated as ovarian cancer-related tumor marker CA125, is a large transmembrane glycoprotein belonging to the mucin family.
• It is thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
• The protein is localized both at the cell membrane and secreted into the extracellular space.
• At the protein level, MUC16 is expressed in corneal and conjunctival epithelia, and it is markedly overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors compared to normal ovarian tissue and adenomas.
• MUC16 is heavily glycosylated and contains multiple disulfide bonds, with a calculated molecular weight of approximately 1519 kDa.
• It is subject to proteomic identification and direct protein sequencing, highlighting its relevance in biomarker research.
• As a member of the mucin family, MUC16's extracellular domain can be shed, releasing CA125 into circulation, which is utilized as a clinical biomarker for ovarian cancer monitoring.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment spanning amino acids 12660–12923 of human CA125 (NP_078966.2). This region lies in the extracellular domain and is suitable for generating antibodies recognizing both membrane-bound and secreted forms.
• For Western blotting (WB), note the large size of MUC16 (~1519 kDa). Consider using low-percentage gradient gels and extended transfer times to ensure efficient detection.
• In ELISA applications, validate the antibody with recombinant CA125 or with lysates from ovarian cancer cell lines (e.g., OVCAR-3) that endogenously overexpress the target.
• Since this is a polyclonal antibody, lot-to-lot consistency can vary; it is advisable to test and titrate each new batch in your specific assay system.
• For optimal results, store and handle the antibody according to the shipping conditions (ice bag) and avoid repeated freeze–thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for high-quality IVD raw materials, from discovery through clinical development. This anti-CA125 (MUC16) polyclonal antibody exemplifies our commitment to supporting ovarian cancer diagnostics research. Beyond antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing—all backed by technical services to streamline your assay development from concept to clinic.