Anti-MUC1 Rabbit Polyclonal Antibody for WB, ELISA - P15941

Core Product Specifications and Parameters

Biological Background: MUC1 Function and Localization

• Mucin-1 (MUC1) is a large, heavily glycosylated transmembrane protein also known as EMA, CA 15-3, and KL-6, with multiple isoforms including a secreted form (isoform Y) expressed in tumor cells.
• The alpha subunit of MUC1 is shed into the extracellular space and mediates cell adhesion, while also providing a protective barrier on epithelial surfaces against pathogens and enzymes.
• The beta subunit anchors to the cell membrane and participates in intracellular signaling via phosphorylation and protein-protein interactions, modulating ERK, SRC, and NF-kappa-B pathways, and regulating TP53-mediated transcription.
• MUC1 localizes to the apical cell membrane of epithelial cells but can also be found secreted, in the cytoplasm, and in the nucleus, depending on the isoform and cleavage state.
• It is prominently expressed in airway passages, breast, and uterus epithelia, and is overexpressed in epithelial tumors (e.g., breast, ovarian) as well as in activated T-cells, making it a key cancer biomarker.
• Post-translational modifications include autocatalytic cleavage, disulfide bond formation, glycation, lipidation (palmitate), and phosphorylation, all contributing to its diverse functions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 50–150 of human MUC1 (P15941). This region is present in multiple isoforms and may allow detection of both full-length and cleaved products in Western blot.
• For WB, consider using reducing or non-reducing conditions depending on whether epitope recognition requires disulfide bond integrity. Due to heavy glycosylation, observed molecular weight may differ from the theoretical 122 kDa.
• Validate antibody performance in your specific sample system; typical positive controls include breast cancer cell lines (e.g., MCF-7) or tissues with high epithelial expression.
• For ELISA, assess linearity and sensitivity across a range of recombinant MUC1 or native antigen concentrations; cross-reactivity with mouse and rat species may simplify translational studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and researchers to streamline procurement of validated IVD antibodies like this anti-MUC1 polyclonal antibody. In the mucin and epithelial tumor biomarker space, we offer complementary monoclonal antibodies, antibody pairs for sandwich assays, and bulk ancillary reagents—removing barriers from target selection to final kit assembly. Tell us your project requirements, and we’ll source or develop the right raw materials for your clinical or translational workflow.