Anti-MUC1 Polyclonal Antibody for WB, IHC-P, ELISA - P15941

Core Product Specifications and Parameters

Biological Background: Mucin-1 Function and Localization

• Mucin-1 (MUC1) is a large, heavily glycosylated transmembrane protein also known as CA15-3, EMA, and KL-6.
• The alpha subunit mediates cell adhesion and can act as both adhesion and anti-adhesion molecule, providing a protective barrier on epithelial surfaces.
• The beta subunit participates in intracellular signaling through phosphorylation and protein interactions, modulating ERK, SRC, and NF-kappa-B pathways.
• MUC1 is expressed on the apical surface of epithelial cells in tissues including airway, breast, and uterus, and is also found in activated T-cells.
• Overexpression of MUC1, particularly isoform Y, is associated with epithelial tumors such as breast and ovarian cancers.
• Subcellularly, MUC1 localizes to the apical cell membrane, but is also secreted and found in cytoplasm and nucleus.
• It undergoes autocatalytic cleavage, alternative splicing, and post-translational modifications including glycosylation, disulfide bond formation, and palmitoylation.
• MUC1 regulates TP53-mediated transcription and can repress TP53 activity, influencing cell fate in genotoxic stress.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal cytoplasmic tail (amino acids 1182–1255); consider potential detection of the beta subunit or cleavage products in Western blot.
• Predicted molecular weight is ~122 kDa, but heavy glycosylation may cause smearing or higher apparent mass; validate with deglycosylation controls if needed.
• For IHC-P, antigen retrieval may be necessary; validate signal in both normal and tumor tissues to confirm specificity.
• In ELISA, use purified or recombinant MUC1 as coating antigen; note that MUC1 is secreted and can be detected in cell supernatants or biological fluids.
• The antibody cross-reacts with mouse and rat MUC1, enabling use in animal models; include appropriate negative controls to confirm species cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with comprehensive IVD raw-material solutions. For MUC1-related oncology and epithelial biology research, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our one-stop platform simplifies procurement from discovery to clinical application.