Anti-MUC1-C Monoclonal Antibody for WB, IHC-P, ELISA - P15941

Core Product Specifications and Parameters

Biological Background: MUC1 Function and Localization

• Mucin-1 (MUC1) is a large transmembrane glycoprotein also known as breast carcinoma-associated antigen DF3, cancer antigen 15-3 (CA 15-3), episialin, and polymorphic epithelial mucin (PEM). It undergoes autocatalytic cleavage into alpha and beta subunits.
• The alpha subunit has cell adhesive properties and can act as both an adhesion and anti-adhesion molecule, potentially providing a protective layer on epithelial cells against bacterial and enzyme attack.
• The beta subunit contains a C-terminal domain involved in cell signaling, modulating pathways such as ERK, SRC, and NF-kappa-B, and promoting tumor progression.
• In activated T-cells, MUC1 influences the Ras/MAPK pathway, and it regulates TP53-mediated transcription by binding, together with KLF4, to the PE21 promoter element of TP53, thereby repressing TP53 activity and determining cell fate in the genotoxic stress response.
• Subcellular localization includes the apical cell membrane, secreted forms, cytoplasm, and nucleus, reflecting its cleavage and signaling functions.
• Tissue expression: constitutively expressed on the apical surface of epithelial cells in airway passages, breast, and uterus; also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors such as breast and ovarian cancer, and isoform Y is expressed only in tumor cells.
• Post-translational modifications include extensive N- and O-linked glycosylation, phosphorylation, and palmitoylation, contributing to its structural and signaling diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal cytoplasmic domain (amino acids 1156–1255), suggesting the antibody recognizes the MUC1 beta subunit (MUC1-C) and may detect full-length and cleaved forms.
• For WB, consider that the predicted molecular weight is 122 kDa, but due to heavy glycosylation and cleavage, the observed band may appear at higher molecular weights or as multiple bands; reducing conditions and appropriate gel percentage (e.g., 6–8%) are recommended.
• For IHC-P, antigen retrieval (e.g., heat-induced epitope retrieval in citrate buffer) may be required; validate in known positive tissues such as breast or ovarian carcinoma.
• For ELISA, optimize coating antigen and antibody concentration using recombinant MUC1-C or cell lysates; cross-reactivity with mouse and rat allows translational studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material sourcing, offering a comprehensive portfolio of validated antibodies, antigens, and ancillary reagents to accelerate diagnostic development. This anti-MUC1-C monoclonal antibody is a valuable tool for cancer biomarker research and assay development, backed by CamelBio’s capability to supply optimized antibody pairs, bulk monoclonal antibodies, and rare-target raw materials. Whether you need consistent lot-to-lot performance or customized sourcing solutions, CamelBio bridges your research needs with high-quality, reliable products.